TY - JOUR
T1 - A gas chromatography/mass spectrometry assay to measure estradiol, catecholestradiols, and methoxyestradiols in plasma
AU - Zacharia, Lefteris C.
AU - Dubey, Raghvendra K.
AU - Jackson, Edwin K.
PY - 2004/4
Y1 - 2004/4
N2 - We have developed a gas chromatography/mass spectrometry (GC/MS) assay to measure 17β-estradiol (E) and its biologically active metabolites 2-hydroxyestradiol (2OHE) and 4-hydroxyestradiol (4OHE), and 2-methoxyestradiol (2MEOE) and 4-methoxyestradiol (4MEOE) in rat plasma. All analytes are well separated and show a linear relationship between concentration (0.25-5 pg/μl) and signal, and coefficients of variation (CVs) are low. Intra-assay CV for the lowest quality control samples (QCs) (0.375 pg/μl) were on average for 17β-estradiol 20.5%, for 2-hydroxyestradiol 15.6%, for 4-hydroxyestradiol 16.5%, for 2-methoxyestradiol 16.5%, and for 4-methoxyestradiol 11.5%. The inter-assay CVs for the lowest QCs were for 17β-estradiol 12.1%, for 2-hydroxyestradiol 7.1%, for 4-hydroxyestradiol 15.5%, for 2-methoxyestradiol 16.7%, and for 4-methoxyestradiol 9.7%. The highest sensitivity for this assay was observed for hydroxyestradiols followed by the methoxyestradiols and 17β-estradiol. In summary, we describe a convenient, sensitive, and specific assay to measure 17β-estradiol and its biologically active metabolites.
AB - We have developed a gas chromatography/mass spectrometry (GC/MS) assay to measure 17β-estradiol (E) and its biologically active metabolites 2-hydroxyestradiol (2OHE) and 4-hydroxyestradiol (4OHE), and 2-methoxyestradiol (2MEOE) and 4-methoxyestradiol (4MEOE) in rat plasma. All analytes are well separated and show a linear relationship between concentration (0.25-5 pg/μl) and signal, and coefficients of variation (CVs) are low. Intra-assay CV for the lowest quality control samples (QCs) (0.375 pg/μl) were on average for 17β-estradiol 20.5%, for 2-hydroxyestradiol 15.6%, for 4-hydroxyestradiol 16.5%, for 2-methoxyestradiol 16.5%, and for 4-methoxyestradiol 11.5%. The inter-assay CVs for the lowest QCs were for 17β-estradiol 12.1%, for 2-hydroxyestradiol 7.1%, for 4-hydroxyestradiol 15.5%, for 2-methoxyestradiol 16.7%, and for 4-methoxyestradiol 9.7%. The highest sensitivity for this assay was observed for hydroxyestradiols followed by the methoxyestradiols and 17β-estradiol. In summary, we describe a convenient, sensitive, and specific assay to measure 17β-estradiol and its biologically active metabolites.
KW - Catecholestradiols
KW - Gas chromatography
KW - Mass spectrometry
KW - Methoxyestradiols
KW - Negative chemical ionization
UR - http://www.scopus.com/inward/record.url?scp=2442668392&partnerID=8YFLogxK
U2 - 10.1016/j.steroids.2004.01.005
DO - 10.1016/j.steroids.2004.01.005
M3 - Article
C2 - 15183691
AN - SCOPUS:2442668392
VL - 69
SP - 255
EP - 261
JO - Steroids
JF - Steroids
SN - 0039-128X
IS - 4
ER -