TY - JOUR
T1 - A novel strategy to enhance mesenchymal stem cell migration capacity and promote tissue repair in an injury specific fashion
AU - Xinaris, Christodoulos
AU - Morigi, M.
AU - Benedetti, V.
AU - Imberti, B.
AU - Fabricio, A. S.
AU - Squarcina, E.
AU - Benigni, A.
AU - Gagliardini, E.
AU - Remuzzi, G.
PY - 2013
Y1 - 2013
N2 - Mesenchymal stem cells (MSCs) of bone marrow origin appear to be an attractive candidate for cell-based therapies. However, the major barrier to the effective implementation of MSC-based therapies is the lack of specific homing of exogenously infused cells and overall the inability to drive them to the diseased or damaged tissue. In order to circumvent these limitations, we developed a preconditioning strategy to optimize MSC migration efficiency and potentiate their beneficial effect at the site of injury. Initially, we screened different molecules by using an in vitro injury-migration setting, and subsequently, we evaluated the effectiveness of the different strategies in mice with acute kidney injury (AKI). Our results showed that preconditioning of MSCs with IGF-1 before infusion improved cell migration capacity and restored normal renal function after AKI. The present study demonstrates that promoting migration of MSCs could increase their therapeutic potential and indicates a new therapeutic paradigm for organ repair.
AB - Mesenchymal stem cells (MSCs) of bone marrow origin appear to be an attractive candidate for cell-based therapies. However, the major barrier to the effective implementation of MSC-based therapies is the lack of specific homing of exogenously infused cells and overall the inability to drive them to the diseased or damaged tissue. In order to circumvent these limitations, we developed a preconditioning strategy to optimize MSC migration efficiency and potentiate their beneficial effect at the site of injury. Initially, we screened different molecules by using an in vitro injury-migration setting, and subsequently, we evaluated the effectiveness of the different strategies in mice with acute kidney injury (AKI). Our results showed that preconditioning of MSCs with IGF-1 before infusion improved cell migration capacity and restored normal renal function after AKI. The present study demonstrates that promoting migration of MSCs could increase their therapeutic potential and indicates a new therapeutic paradigm for organ repair.
KW - Acute kidney injury (AKI)
KW - Insulin-like growth factor-1 (IGF-1)
KW - Kidney repair
KW - Mesenchymal stem cells (MSCs)
KW - Migration
UR - https://www.scopus.com/pages/publications/84877311784
U2 - 10.3727/096368912X653246
DO - 10.3727/096368912X653246
M3 - Article
C2 - 22889699
AN - SCOPUS:84877311784
SN - 0963-6897
VL - 22
SP - 423
EP - 436
JO - Cell Transplantation
JF - Cell Transplantation
IS - 3
ER -