A protocol for the generation of EPO - Producing neural crest cells from human induced pluripotent stem cells

Angelo Michele Lavecchia; Sara Buttò; Christodoulos Xinaris

doi:10.1016/j.mex.2022.101753

A protocol for the generation of EPO - Producing neural crest cells from human induced pluripotent stem cells

Angelo Michele Lavecchia
, Sara Buttò
, Christodoulos Xinaris

IRCCS Istituto di ricerche farmacologiche Mario Negri - Milano, Bergamo, Ranica

Research output: Contribution to journal › Article › peer-review

Abstract

Insufficient production of erythropoietin (EPO) leads to anaemia. Developing methods for the generation and transplantation of EPO-producing cells would allow scientists to design personalised therapeutic solutions. Here we present a simple and highly reproducible protocol for the generation of neural crest cells (NCCs) that can produce and secrete erythropoiesis-competent EPO in response to hypoxia.

Original language	English
Article number	101753
Journal	MethodsX
Volume	9
DOIs	https://doi.org/10.1016/j.mex.2022.101753
Publication status	Published - Jan 2022
Externally published	Yes

Keywords

Anaemia
Erythropoietin
Human induced pluripotent stem cells
Neural crest cells
XXXXX

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10.1016/j.mex.2022.101753

Cite this

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abstract = "Insufficient production of erythropoietin (EPO) leads to anaemia. Developing methods for the generation and transplantation of EPO-producing cells would allow scientists to design personalised therapeutic solutions. Here we present a simple and highly reproducible protocol for the generation of neural crest cells (NCCs) that can produce and secrete erythropoiesis-competent EPO in response to hypoxia.",

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AU - Xinaris, Christodoulos

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N2 - Insufficient production of erythropoietin (EPO) leads to anaemia. Developing methods for the generation and transplantation of EPO-producing cells would allow scientists to design personalised therapeutic solutions. Here we present a simple and highly reproducible protocol for the generation of neural crest cells (NCCs) that can produce and secrete erythropoiesis-competent EPO in response to hypoxia.

AB - Insufficient production of erythropoietin (EPO) leads to anaemia. Developing methods for the generation and transplantation of EPO-producing cells would allow scientists to design personalised therapeutic solutions. Here we present a simple and highly reproducible protocol for the generation of neural crest cells (NCCs) that can produce and secrete erythropoiesis-competent EPO in response to hypoxia.

KW - Anaemia

KW - Erythropoietin

KW - Human induced pluripotent stem cells

KW - Neural crest cells

KW - XXXXX

UR - https://www.scopus.com/pages/publications/85132510979

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DO - 10.1016/j.mex.2022.101753

M3 - Article

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ER -

A protocol for the generation of EPO - Producing neural crest cells from human induced pluripotent stem cells

Abstract

Keywords

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