TY - JOUR
T1 - Activation of prosurvival signaling pathways during the memory phase of volatile anesthetic preconditioning in human myocardium
T2 - A pilot study
AU - Mellidis, Kyriakos
AU - Ordodi, Valentin
AU - Galatou, Eleftheria
AU - Sǎndesc, Dorel
AU - Bubenek, Şerban
AU - Duicu, Oana
AU - Muntean, Danina
AU - Lazou, Antigone
PY - 2014/3
Y1 - 2014/3
N2 - According to a compelling body of evidence anesthetic preconditioning (APC) attenuates the deleterious consequences of ischemia-reperfusion and protects the heart through a mechanism similar to ischemic preconditioning. The present study was purported to investigate the intracellular signaling pathways activated in human myocardium in response to a preconditioning protocol with two different volatile anesthetics, namely isoflurane and sevoflurane. To this aim, phosphorylation of PKCα and -δ, ERK1/2, Akt, and GSK3β was determined at the end of the APC protocol, in human atrial samples harvested from patients undergoing open-heart surgery. The results demonstrate that preconditioning with volatile anesthetics triggers the activation of PKCδ and -α isoforms and of prosurvival kinases, ERK1/2, and Akt, while inhibiting their downstream target GSK3β during the memory phase.
AB - According to a compelling body of evidence anesthetic preconditioning (APC) attenuates the deleterious consequences of ischemia-reperfusion and protects the heart through a mechanism similar to ischemic preconditioning. The present study was purported to investigate the intracellular signaling pathways activated in human myocardium in response to a preconditioning protocol with two different volatile anesthetics, namely isoflurane and sevoflurane. To this aim, phosphorylation of PKCα and -δ, ERK1/2, Akt, and GSK3β was determined at the end of the APC protocol, in human atrial samples harvested from patients undergoing open-heart surgery. The results demonstrate that preconditioning with volatile anesthetics triggers the activation of PKCδ and -α isoforms and of prosurvival kinases, ERK1/2, and Akt, while inhibiting their downstream target GSK3β during the memory phase.
KW - Kinases
KW - Memory phase
KW - Preconditioning
KW - Volatile anesthetics
UR - http://www.scopus.com/inward/record.url?scp=84893827996&partnerID=8YFLogxK
U2 - 10.1007/s11010-013-1910-5
DO - 10.1007/s11010-013-1910-5
M3 - Article
C2 - 24276754
AN - SCOPUS:84893827996
SN - 0300-8177
VL - 388
SP - 195
EP - 201
JO - Molecular and Cellular Biochemistry
JF - Molecular and Cellular Biochemistry
IS - 1-2
ER -