Assessment of apoptosis in human breast tissue using an antibody against the active form of caspase 3: Relation to tumour histopathological characteristics

I. Hadjiloucas, A. P. Gilmore, N. J. Bundred, C. H. Streuli

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Apoptosis is of important significance in the pathogenesis of cancer. Many methods are available for the measurement of apoptosis but the 'gold standard' is to identify apoptotic cells by their morphological features using microscopy. Caspase 3 is a cytosolic enzyme that is activated only in cells committed to undergo apoptosis. The activation of caspase 3 precedes the development of the classical morphological features of apoptosis. Using immunohistochemistry with an antibody against the active form of caspase 3, the apoptotic index (Al) was measured in 116 samples of human breast tissue (22 normal/benign and 94 invasive carcinomas). The Al obtained by measuring caspase activation has a strong correlation with the Al derived by morphological assessment (r = 0.736, P < 0.01). The Al is higher in the invasive group than in the benign group (P = 0.008), and in invasive cancer high Al is associated with high tumour grade (P = 0.013), positive node status (P < 0.001) and negative steroid receptor status (P = 0.001 for ER; P = 0.004 for PR). No significant association is observed between Al and tumour size. Measurement of apoptosis by immunohistochemistry using an antibody against the active form of caspase 3 is therefore reliable and correlates strongly with morphological assessment.

Original languageEnglish
Pages (from-to)1522-1526
Number of pages5
JournalBritish Journal of Cancer
Volume85
Issue number10
DOIs
Publication statusPublished - 16 Nov 2001

Keywords

  • Apoptosis
  • Breast cancer
  • Caspase 3
  • Mammary gland

Fingerprint Dive into the research topics of 'Assessment of apoptosis in human breast tissue using an antibody against the active form of caspase 3: Relation to tumour histopathological characteristics'. Together they form a unique fingerprint.

  • Cite this