TY - JOUR
T1 - Cationic star polymer siRNA transfectants interconnected with a piperazine-based cationic cross-linker
AU - Pafiti, Kyriaki S.
AU - Patrickios, Costas S.
AU - Georgiou, Theoni K.
AU - Yamasaki, Edna N.
AU - Mastroyiannopoulos, Nikolaos P.
AU - Phylactou, Leonidas A.
PY - 2012/8
Y1 - 2012/8
N2 - Cationic star polymers of 2-(dimethylamino)ethyl methacrylate (DMAEMA) were prepared via the "arm-first" method under group transfer polymerization (GTP) conditions, and were interconnected using a novel, hydrophilic, positively ionizable dimethacrylate cross-linker which is essentially the cyclic dimer of DMAEMA, thus ensuring that the building units of the arms and the core of the star polymers were identical. After their physicochemical characterization, these star-like polyamines were evaluated for their ability to transfer small interfering RNA (siRNA) to murine myoblast cells. Suppression efficiency was found to increase with polymer loading and star branch size, attaining sufficiently high values, comparable to that observed with standard non-viral siRNA transfection systems, while cytotoxicity was kept reasonably low.
AB - Cationic star polymers of 2-(dimethylamino)ethyl methacrylate (DMAEMA) were prepared via the "arm-first" method under group transfer polymerization (GTP) conditions, and were interconnected using a novel, hydrophilic, positively ionizable dimethacrylate cross-linker which is essentially the cyclic dimer of DMAEMA, thus ensuring that the building units of the arms and the core of the star polymers were identical. After their physicochemical characterization, these star-like polyamines were evaluated for their ability to transfer small interfering RNA (siRNA) to murine myoblast cells. Suppression efficiency was found to increase with polymer loading and star branch size, attaining sufficiently high values, comparable to that observed with standard non-viral siRNA transfection systems, while cytotoxicity was kept reasonably low.
KW - Gene transfer
KW - Group transfer polymerization
KW - Piperazine cross-linker
KW - siRNA delivery systems
KW - Small interfering RNA
KW - Star polymers
UR - http://www.scopus.com/inward/record.url?scp=84863989425&partnerID=8YFLogxK
U2 - 10.1016/j.eurpolymj.2012.05.008
DO - 10.1016/j.eurpolymj.2012.05.008
M3 - Article
AN - SCOPUS:84863989425
SN - 0014-3057
VL - 48
SP - 1422
EP - 1430
JO - European Polymer Journal
JF - European Polymer Journal
IS - 8
ER -