Cationic star polymer siRNA transfectants interconnected with a piperazine-based cationic cross-linker

Kyriaki S. Pafiti, Costas S. Patrickios, Theoni K. Georgiou, Edna N. Yamasaki, Nikolaos P. Mastroyiannopoulos, Leonidas A. Phylactou

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Cationic star polymers of 2-(dimethylamino)ethyl methacrylate (DMAEMA) were prepared via the "arm-first" method under group transfer polymerization (GTP) conditions, and were interconnected using a novel, hydrophilic, positively ionizable dimethacrylate cross-linker which is essentially the cyclic dimer of DMAEMA, thus ensuring that the building units of the arms and the core of the star polymers were identical. After their physicochemical characterization, these star-like polyamines were evaluated for their ability to transfer small interfering RNA (siRNA) to murine myoblast cells. Suppression efficiency was found to increase with polymer loading and star branch size, attaining sufficiently high values, comparable to that observed with standard non-viral siRNA transfection systems, while cytotoxicity was kept reasonably low.

Original languageEnglish
Pages (from-to)1422-1430
Number of pages9
JournalEuropean Polymer Journal
Volume48
Issue number8
DOIs
Publication statusPublished - Aug 2012

Keywords

  • Gene transfer
  • Group transfer polymerization
  • Piperazine cross-linker
  • siRNA delivery systems
  • Small interfering RNA
  • Star polymers

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