TY - JOUR
T1 - Clinical reactivation during lamivudine treatment correlates with mutations in the precore/core promoter and polymerase regions of hepatitis B virus in patients with anti-hepatitis B e-positive chronic hepatitis
AU - Marrone, A.
AU - Zampino, R.
AU - Karayannis, P.
AU - Cirillo, G.
AU - Cesaro, G.
AU - Guerrera, B.
AU - Ricciotti, R.
AU - Miraglia Del Giudice, E.
AU - Utili, R.
AU - Adinolfi, L. E.
AU - Ruggiero, G.
PY - 2005/10/15
Y1 - 2005/10/15
N2 - Background: Drug-resistant mutants may emerge in patients with chronic hepatitis B receiving lamivudine therapy. Aim: To evaluate whether different viral mutational patterns may be associated with clinical reactivation during lamivudine treatment in patients with chronic B hepatitis. Methods: Eight anti-hepatitis B e-positive patients with (group A) and 14 patients without clinical exacerbation (five anti-hepatitis B e-positive, group B1; nine hepatitis B e antigen-positive, group B2) during lamivudine treatment were investigated. Results: 'Polymerase region': M204V/1 variants were found in all group A patients, but in none of group B1 (P = 0.0007) and in four of nine of group B2 (44%; P = 0.02) patients. The L180M substitution was detected in four of eight (50%) of group A and in none of groups B1 and B2. 'Core promoter': the double basic core promoter (A1762T/G1764A) variant was detected in seven of eight (87%) of group A and in one of five (20%; P = 0.03) of group B1 and one of nine (11%; P = 0.002) of group B2 patients. 'Precore': the G1896A stop codon mutation was present in seven of eight (87%) of group A and in zero of five (P = 0.004) of group B1 and one of nine (11%; P -0.002) of group B2. Conclusions: Different mutational patterns were observed in the lamivudine-treated patients with and without exacerbation. There was an association of the basic core promoter and stop codon mutations with lamivudine resistance in patients with disease exacerbation.
AB - Background: Drug-resistant mutants may emerge in patients with chronic hepatitis B receiving lamivudine therapy. Aim: To evaluate whether different viral mutational patterns may be associated with clinical reactivation during lamivudine treatment in patients with chronic B hepatitis. Methods: Eight anti-hepatitis B e-positive patients with (group A) and 14 patients without clinical exacerbation (five anti-hepatitis B e-positive, group B1; nine hepatitis B e antigen-positive, group B2) during lamivudine treatment were investigated. Results: 'Polymerase region': M204V/1 variants were found in all group A patients, but in none of group B1 (P = 0.0007) and in four of nine of group B2 (44%; P = 0.02) patients. The L180M substitution was detected in four of eight (50%) of group A and in none of groups B1 and B2. 'Core promoter': the double basic core promoter (A1762T/G1764A) variant was detected in seven of eight (87%) of group A and in one of five (20%; P = 0.03) of group B1 and one of nine (11%; P = 0.002) of group B2 patients. 'Precore': the G1896A stop codon mutation was present in seven of eight (87%) of group A and in zero of five (P = 0.004) of group B1 and one of nine (11%; P -0.002) of group B2. Conclusions: Different mutational patterns were observed in the lamivudine-treated patients with and without exacerbation. There was an association of the basic core promoter and stop codon mutations with lamivudine resistance in patients with disease exacerbation.
UR - http://www.scopus.com/inward/record.url?scp=26944437038&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2036.2005.02653.x
DO - 10.1111/j.1365-2036.2005.02653.x
M3 - Article
C2 - 16197491
AN - SCOPUS:26944437038
SN - 0269-2813
VL - 22
SP - 707
EP - 714
JO - Alimentary Pharmacology and Therapeutics
JF - Alimentary Pharmacology and Therapeutics
IS - 8
ER -