Clusters of imipenem-resistant acinetobacter baumannii clones producing different carbapenemases in an intensive care unit

A. Tsakris, A. Ikonomidis, A. Poulou, N. Spanakis, D. Vrizas, M. Diomidous, S. Pournaras, F. Markou

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Abstract

During a 2-year period (April 2005-March 2007), 31 intensive care unit (ICU) patients in a Greek hospital were infected or colonised with imipenem-resistant isolates of Acinetobacter baumannii. Twelve patients died, with imipenem-resistant A. baumannii infection contributing to the death of seven patients. The 31 representative A. baumannii isolates were multidrug-resistant and clustered in four distinct clones, each of which contained different carbapenemase genes: Clone I was predominant and contained blaVIM-1, blaOXA-58 and the intrinsic blaOXA-66 gene; clone II contained blaVIM-4, blaOXA-58 and the intrinsic blaOXA-69gene; clone III contained blaOXA-58 and the intrinsic blaOXA-69 gene; and clone IV contained only the intrinsic blaOXA-66 gene. ISAba1 was not associated with the intrinsic blaOXA-51-like alleles, whereas ISAba3 was found upstream and downstream of blaOXA-58 in isolates of clone I, and upstream of blaOXA-58 in isolates of clone III, but was not detected in isolates of clone II. PCR, curing and hybridisation experiments indicated that the blaVIM alleles were chromosomally located, whereas the blaOXA-58 alleles were plasmid-located. This study provides the first description of the clonal spread of multidrug-resistant A. baumannii isolates carrying blaVIM-1 and blaVIM-4 metallo-β-lactamase genes, and revealed that distinct carbapenem-resistant A. baumannii clusters bearing different carbapenemase genes may emerge and cause severe infections, even in a well-defined regional hospital setting.

Original languageEnglish
Pages (from-to)588-594
Number of pages7
JournalClinical Microbiology and Infection
Volume14
Issue number6
DOIs
Publication statusPublished - 2008

Keywords

  • Acinetobacter baumannii
  • Carbapenemases
  • Epidemiology
  • OXA-51
  • OXA-58
  • VIM metallo-β-lactamases

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    Tsakris, A., Ikonomidis, A., Poulou, A., Spanakis, N., Vrizas, D., Diomidous, M., Pournaras, S., & Markou, F. (2008). Clusters of imipenem-resistant acinetobacter baumannii clones producing different carbapenemases in an intensive care unit. Clinical Microbiology and Infection, 14(6), 588-594. https://doi.org/10.1111/j.1469-0691.2008.01996.x