Detection and significance of a G1862T variant of hepatitis B virus in Chinese patients with fulminant hepatitis

Jinlin Hou, Yulong Lin, Jenny Waters, Zhanhui Wang, Jia Min, Huiyu Liao, Jiaji Jiang, Jinjun Chen, Kangxian Luo, Peter Karayiannis

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Abstract

The prevalence of a G1862T variant of hepatitis B virus (HBV) has been investigated in patients with fulminant hepatitis and chronic liver disease, using primer mismatch amplification, followed by restriction fragment length polymorphism analysis. This variant was five times more common in patients with fulminant hepatitis (13.7%, 7 of 52) than in chronic carriers (2.5%, 2 of 81). The G → T substitution at position 1862 leads to an amino acid change in codon 17 of the precore protein of the virus, which is part of a signal peptidase recognition motif. Variants with this mutation were only seen in patients infected with genotype B. In vitro translation experiments showed that this variant has greatly reduced capacity to produce hepatitis B e antigen (HBeAg) from its precore protein precursor. Furthermore, 88.5% of patients with fulminant hepatitis had mutations that are known to be associated with abrogated or reduced production of HBeAg. This suggests that, following HBV infection, the absence or reduced amounts of HBeAg may be a contributing factor in fulminant disease.

Original languageEnglish
Pages (from-to)2291-2298
Number of pages8
JournalJournal of General Virology
Volume83
Issue number9
Publication statusPublished - Sep 2002

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    Hou, J., Lin, Y., Waters, J., Wang, Z., Min, J., Liao, H., Jiang, J., Chen, J., Luo, K., & Karayiannis, P. (2002). Detection and significance of a G1862T variant of hepatitis B virus in Chinese patients with fulminant hepatitis. Journal of General Virology, 83(9), 2291-2298.