Detection of cryptosporidium oocysts in green mussels [perna viridis from shell-fish markets of Thailand

Mussels filter large volumes of water and can concentrate pathogenic organisms, which may act as potential vehicles of transmission to the consumer. A survey study was earned out to investigate the presence of Cryptosporidium protozoan parasites in green mussels [Pema viridis), the smussles pecies most destined for consumption in Thailand. In total, 50 samples were examined from Bangkok (n = 24) and Samut Prakan (n = 32) a wholesale shell-fish markers located at the mouth of the Chao Phraya River. The market for green mussels was closed to the mussel culture placed along the coastal line and this localization may have significant economical impact if the mussels' cultures are found contaminated. Cryptosporidium spp. oocysts were detected by the immunofluorescence antibody method (IFA) in 12.5 % of the samples examined. The detection of Cryptosporidium oocysts in green mussels' population of Samut Prakan was higher (15.0%) than in Bangkok market (8.3 %). These differences in positive samples from the two locations may be caused by physical, ecological and anthropogenic conditions. This could relay to different contamination levels of marine water by Cryptosporidium oocysts and consequently to contamination of harvested shellfish populations. The results demonstrate that the Cryptosporidium spp. oocysts were found indigenous in mussels from the coastal line of Thailand, indicating that mussels may act as a reservoir of Cryptosporidium foodbome infections for humans.