Development and preliminary evaluation of a loop-mediated isothermal amplification procedure for sensitive detection of Cryptosporidium oocysts in fecal and water samples

Panagiotis Karanis; Oriel Thekisoe; Klytaimnistra Kiouptsi; Jerry Ongerth; Ikuo Igarashi; Noborou Inoue

doi:10.1128/AEM.01152-07

Development and preliminary evaluation of a loop-mediated isothermal amplification procedure for sensitive detection of Cryptosporidium oocysts in fecal and water samples

Panagiotis Karanis
, Oriel Thekisoe
, Klytaimnistra Kiouptsi
, Jerry Ongerth
, Ikuo Igarashi
, Noborou Inoue

Research output: Contribution to journal › Article › peer-review

Abstract

A loop-mediated isothermal amplification (LAMP) procedure for the detection of Cryptosporidium in environmental and fecal samples was developed and evaluated. This is the first demonstration of LAMP applied to detection of Cryptosporidium. Due to its specificity and simplicity, the method could become a useful diagnostic tool for epidemiologic studies of Cryptosporidium presence.

Original language	English
Pages (from-to)	5660-5662
Number of pages	3
Journal	Applied and Environmental Microbiology
Volume	73
Issue number	17
DOIs	https://doi.org/10.1128/AEM.01152-07
Publication status	Published - Sept 2007
Externally published	Yes

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10.1128/AEM.01152-07

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title = "Development and preliminary evaluation of a loop-mediated isothermal amplification procedure for sensitive detection of Cryptosporidium oocysts in fecal and water samples",

abstract = "A loop-mediated isothermal amplification (LAMP) procedure for the detection of Cryptosporidium in environmental and fecal samples was developed and evaluated. This is the first demonstration of LAMP applied to detection of Cryptosporidium. Due to its specificity and simplicity, the method could become a useful diagnostic tool for epidemiologic studies of Cryptosporidium presence.",

author = "Panagiotis Karanis and Oriel Thekisoe and Klytaimnistra Kiouptsi and Jerry Ongerth and Ikuo Igarashi and Noborou Inoue",

year = "2007",

month = sep,

doi = "10.1128/AEM.01152-07",

language = "English",

volume = "73",

pages = "5660--5662",

journal = "Applied and Environmental Microbiology",

issn = "0099-2240",

publisher = "American Society for Microbiology",

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Development and preliminary evaluation of a loop-mediated isothermal amplification procedure for sensitive detection of Cryptosporidium oocysts in fecal and water samples. / Karanis, Panagiotis; Thekisoe, Oriel; Kiouptsi, Klytaimnistra et al.
In: Applied and Environmental Microbiology, Vol. 73, No. 17, 09.2007, p. 5660-5662.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Development and preliminary evaluation of a loop-mediated isothermal amplification procedure for sensitive detection of Cryptosporidium oocysts in fecal and water samples

AU - Karanis, Panagiotis

AU - Thekisoe, Oriel

AU - Kiouptsi, Klytaimnistra

AU - Ongerth, Jerry

AU - Igarashi, Ikuo

AU - Inoue, Noborou

PY - 2007/9

Y1 - 2007/9

N2 - A loop-mediated isothermal amplification (LAMP) procedure for the detection of Cryptosporidium in environmental and fecal samples was developed and evaluated. This is the first demonstration of LAMP applied to detection of Cryptosporidium. Due to its specificity and simplicity, the method could become a useful diagnostic tool for epidemiologic studies of Cryptosporidium presence.

AB - A loop-mediated isothermal amplification (LAMP) procedure for the detection of Cryptosporidium in environmental and fecal samples was developed and evaluated. This is the first demonstration of LAMP applied to detection of Cryptosporidium. Due to its specificity and simplicity, the method could become a useful diagnostic tool for epidemiologic studies of Cryptosporidium presence.

UR - https://www.scopus.com/pages/publications/34548478794

U2 - 10.1128/AEM.01152-07

DO - 10.1128/AEM.01152-07

M3 - Article

C2 - 17616628

AN - SCOPUS:34548478794

SN - 0099-2240

VL - 73

SP - 5660

EP - 5662

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 17

ER -

Development and preliminary evaluation of a loop-mediated isothermal amplification procedure for sensitive detection of Cryptosporidium oocysts in fecal and water samples

Abstract

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