TY - JOUR
T1 - Effect of in vitro gastric and duodenal digestion on the allergenicity of grape lipid transfer protein
AU - Vassilopoulou, Emilia
AU - Rigby, Neil
AU - Moreno, F. Javier
AU - Zuidmeer, Laurian
AU - Akkerdaas, Jaap
AU - Tassios, Ioannis
AU - Papadopoulos, Nikos G.
AU - Saxoni-Papageorgiou, Photini
AU - van Ree, Ronald
AU - Mills, Clare
PY - 2006/8
Y1 - 2006/8
N2 - Background: Severe grape allergy has been linked to lipid transfer protein (LTP) sensitization. LTPs are known to be resistant to pepsin digestion, although the effect of gastroduodenal digestion on its allergenicity has not been reported. Objective: We sought to investigate the effect of gastric and gastroduodenal digestion on the allergenic activity of grape LTP. Methods: The proteolytic stability of grape LTP was investigated by using an in vitro model of gastrointestinal digestion. The allergenicity of LTP and its digesta was assessed in vitro by means of IgE immunoblotting, RASTs, and in vivo skin prick tests in the same patients with grape allergy. Results: Grape LTP was resistant to gastric digestion, and yielded a 6000-d relative molecular mass C-terminally trimmed fragment after duodenal digestion. This fragment retained the in vitro IgE reactivity of the intact protein. Inclusion of phosphatidylcholine during gastric digestion protected the LTP to a limited extent against digestion. Digestion did not affect the in vivo (skin prick test) biologic activity of LTP. Conclusion: The allergenic activity of grape LTP was highly resistant to in vitro digestion. This property might facilitate sensitization through the gastrointestinal tract and might also potentiate the ability of LTPs to elicit severe allergic reactions in sensitized individuals. Clinical implications: Purified natural allergens will facilitate the development of component-resolved diagnostic approaches, including allergen chips. This study contributes to our understanding of the role digestion plays in symptom elicitation in true food allergy.
AB - Background: Severe grape allergy has been linked to lipid transfer protein (LTP) sensitization. LTPs are known to be resistant to pepsin digestion, although the effect of gastroduodenal digestion on its allergenicity has not been reported. Objective: We sought to investigate the effect of gastric and gastroduodenal digestion on the allergenic activity of grape LTP. Methods: The proteolytic stability of grape LTP was investigated by using an in vitro model of gastrointestinal digestion. The allergenicity of LTP and its digesta was assessed in vitro by means of IgE immunoblotting, RASTs, and in vivo skin prick tests in the same patients with grape allergy. Results: Grape LTP was resistant to gastric digestion, and yielded a 6000-d relative molecular mass C-terminally trimmed fragment after duodenal digestion. This fragment retained the in vitro IgE reactivity of the intact protein. Inclusion of phosphatidylcholine during gastric digestion protected the LTP to a limited extent against digestion. Digestion did not affect the in vivo (skin prick test) biologic activity of LTP. Conclusion: The allergenic activity of grape LTP was highly resistant to in vitro digestion. This property might facilitate sensitization through the gastrointestinal tract and might also potentiate the ability of LTPs to elicit severe allergic reactions in sensitized individuals. Clinical implications: Purified natural allergens will facilitate the development of component-resolved diagnostic approaches, including allergen chips. This study contributes to our understanding of the role digestion plays in symptom elicitation in true food allergy.
KW - basophil histamine release
KW - duodenal digestion
KW - food allergy
KW - gastric digestion
KW - Grape
KW - lipid transfer protein
KW - phosphatidylcholine
KW - skin prick testing
UR - http://www.scopus.com/inward/record.url?scp=33746483484&partnerID=8YFLogxK
U2 - 10.1016/j.jaci.2006.04.057
DO - 10.1016/j.jaci.2006.04.057
M3 - Article
C2 - 16890774
AN - SCOPUS:33746483484
SN - 0091-6749
VL - 118
SP - 473
EP - 480
JO - Journal of Allergy and Clinical Immunology
JF - Journal of Allergy and Clinical Immunology
IS - 2
ER -