Ethanol increases GABA release in the embryonic avian retina

Fernanda Pohl-Guimarães, Karin da Costa Calaza, Edna Nanami Yamasaki, Regina Célia Cussa Kubrusly, Ricardo Augusto de Melo Reis

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Several mechanisms underlying ethanol action in GABAergic synapses have been proposed, one of these mechanisms is on GABA release. Here, we report that in ovo exposure to ethanol induces an increase on GABA release in the embryonic chick retina. Eleven-day-old chick embryos (E11) received an injection of either phosphate buffer saline (PBS) or ethanol (10%, v/v, diluted in PBS), and were allowed to develop until E16. A single glutamate stimulus (2 mM) showed approximately a 40% increase on GABA release in E16 retinas when compared to controls. The effect was dependent on NMDA receptors and GAD65 mRNA levels, which were increased following the ethanol treatment. However, the numbers of GABA-, GAD-, and NR1-immunoreactive cells, and the expression levels of these proteins, were not affected. We conclude that ethanol treatment at a time point when synapses are being formed during development selectively increases GABA release in the retina via a NMDA receptor-dependent process.

Original languageEnglish
Pages (from-to)189-194
Number of pages6
JournalInternational Journal of Developmental Neuroscience
Volume28
Issue number2
DOIs
Publication statusPublished - Apr 2010

Keywords

  • Development
  • Ethanol
  • GABA
  • NMDA
  • Retina

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