Evaluation of three flocculation methods for the purification of Cryptosporidium parvum oocysts from water samples

Aims: Evaluation of three flocculation methods for the purification of Cryptosporidium parvum oocysts from tap water. Methods and Results: Ferric sulphate, aluminium sulphate and calcium carbonate were compared for their recovery efficiency of C. parvum oocysts from tap water. Lower mean recovery was achieved by calcium carbonate (38.8%) compared with ferric sulphate (61.5%) and aluminium sulphate (58.1%) for the recovery of 2.5 × 10⁵ oocysts l^-1; 2.5 oocysts l^-1 and 1 oocyst l^-1 were adequately purified using ferric sulphate flocculation. In vitro excystation experiments showed that ferric sulphate flocculation does not markedly reduce the viability of oocysts. Conclusions: Ferric sulphate flocculation is a simple and effective tool for the purification of C. parvum oocysts from tap water. Significance and Impact of the Study: The high recovery rates and low impact on oocyst viability provided by ferric sulphate flocculation might be useful for the detection of Cryptosporidium oocysts in environmental water samples.