TY - JOUR
T1 - Flagellin gene (fliC) of Thermus thermophilus HB8
T2 - Characterization of its product and involvement to flagella assembly and microbial motility
AU - Papaneophytou, Christos P.
AU - Papi, Rigini M.
AU - Pantazaki, Anastasia A.
AU - Kyriakidis, Dimitrios A.
PY - 2012/6
Y1 - 2012/6
N2 - Thermus thermophilus HB8 flagellin protein (FliC) is encoded by the TTHC004 (fliC) gene, which is located in the pTT8 plasmid of the bacterium. Flagellinmonomer and flagella fibres were isolated from a culture of T. thermophilus grown in rich medium, or in mineral salt medium with sodium gluconate as the carbon source. Western blot immunodetection with anti-FliC revealed a stable complex (FliC) 1(FliS) 2 of flagellin (FliC, 27.7 kDa) with a homodimer of FliS (FliS, 18.2 kDa) that are encoded by TTHC004 and TTHC003 genes, respectively. The complex is dissociable at low pHs and/or by heat treatment. Glycan staining of purified flagella and treatment with N-glycosidase F suggested that flagellin of T. thermophilus is a glycosylated protein. Size exclusion chromatography revealed that flagellar filaments (FliC) have a molecular mass higher than 200 kDa. The formation of flagella is enhanced after prolonged cultivation time where phosphate and other nutrient were depleted, giving in the bacterium considerable swimming motility in low viscosity media.
AB - Thermus thermophilus HB8 flagellin protein (FliC) is encoded by the TTHC004 (fliC) gene, which is located in the pTT8 plasmid of the bacterium. Flagellinmonomer and flagella fibres were isolated from a culture of T. thermophilus grown in rich medium, or in mineral salt medium with sodium gluconate as the carbon source. Western blot immunodetection with anti-FliC revealed a stable complex (FliC) 1(FliS) 2 of flagellin (FliC, 27.7 kDa) with a homodimer of FliS (FliS, 18.2 kDa) that are encoded by TTHC004 and TTHC003 genes, respectively. The complex is dissociable at low pHs and/or by heat treatment. Glycan staining of purified flagella and treatment with N-glycosidase F suggested that flagellin of T. thermophilus is a glycosylated protein. Size exclusion chromatography revealed that flagellar filaments (FliC) have a molecular mass higher than 200 kDa. The formation of flagella is enhanced after prolonged cultivation time where phosphate and other nutrient were depleted, giving in the bacterium considerable swimming motility in low viscosity media.
KW - Flagella assembly
KW - Flagellin
KW - fliC gene
KW - Motility
KW - T. Thermophilus HB8
UR - http://www.scopus.com/inward/record.url?scp=84863841770&partnerID=8YFLogxK
U2 - 10.1007/s00253-012-3913-7
DO - 10.1007/s00253-012-3913-7
M3 - Article
C2 - 22354365
AN - SCOPUS:84863841770
SN - 0175-7598
VL - 94
SP - 1265
EP - 1277
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
IS - 5
ER -