Genetic diversity among accessions of an ancient olive variety of Cyprus

Georgios Banilas, John Minas, Costas Gregoriou, Cathrine Demoliou, Anna Kourti, Polydefkis Hatzopoulos

Research output: Contribution to journalArticlepeer-review


To evaluate germplasm variability and to discriminate between accessions of 'Ladolia', an ancient olive variety of Cyprus, different accessions from a germplasm collection were screened with 11 selected oligonucleotide primers in RAPD-PCRs. A total of 49 polymorphic markers were scored, the combination of which resulted in 70 distinct electrophoretic patterns. Based on either unique or combined patterns, all accessions were identified. Seven genotype-specific markers were detected. One RAPD marker could distinguish accessions according to fruit size. Genetic similarities between accessions, estimated using the Dice similarity coefficient, indicated a high degree of genetic diversity among 'Ladolia' accessions. Genetic relationships were estimated by the unweighted pair-group method with arithmetic averaging (UPGMA) and principal components analysis (PCA). Three main groups of accessions were detected. The first group was generally composed of accessions with small-sized fruits and could be further divided into two sub-groups. According to PCA, most accessions with medium- or large-sized fruits were clustered together. Our results support previous observations suggesting that 'Ladolia' is actually a highly variable mixture of genetically distinct landraces.

Original languageEnglish
Pages (from-to)370-376
Number of pages7
Issue number3
Publication statusPublished - Jun 2003


  • DNA polymorphism
  • Germplasm variability
  • Olea europaea
  • RAPD markers


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