Giardia and Cryptosporidium spp. dissemination during wastewater treatment and comparative detection via immunofluorescence assay (IFA), nested polymerase chain reaction (nested PCR) and loop mediated isothermal amplification (LAMP)

Carmen Gallas-Lindemann; Isaia Sotiriadou; Judit Plutzer; Michael J. Noack; Mohammad Reza Mahmoudi; Panagiotis Karanis

doi:10.1016/j.actatropica.2016.02.005

Giardia and Cryptosporidium spp. dissemination during wastewater treatment and comparative detection via immunofluorescence assay (IFA), nested polymerase chain reaction (nested PCR) and loop mediated isothermal amplification (LAMP)

Carmen Gallas-Lindemann
, Isaia Sotiriadou
, Judit Plutzer
, Michael J. Noack
, Mohammad Reza Mahmoudi
, Panagiotis Karanis

Research output: Contribution to journal › Article › peer-review

Abstract

Environmental water samples from the Lower Rhine area in Germany were investigated via immunofluorescence assays (IFAs), nested polymerase chain reaction (nested PCR) and loop-mediated isothermal amplification (LAMP) to detect the presence of Giardia spp. (n = 185) and Cryptosporidium spp. (n = 227). The samples were concentrated through filtration or flocculation, and oocysts were purified via centrifugation through a sucrose density gradient. For all samples, IFA was performed first, followed by DNA extraction for the nested PCR and LAMP assays. Giardia cysts were detected in 105 samples (56.8%) by IFA, 62 samples (33.5%) by nested PCR and 79 samples (42.7%) by LAMP. Cryptosporidium spp. were detected in 69 samples (30.4%) by IFA, 95 samples (41.9%) by nested PCR and 99 samples (43.6%) by LAMP. According to these results, the three detection methods are complementary for monitoring Giardia and Cryptosporidium in environmental waters.

Original language	English
Pages (from-to)	43-51
Number of pages	9
Journal	Acta Tropica
Volume	158
DOIs	https://doi.org/10.1016/j.actatropica.2016.02.005
Publication status	Published - 1 Jun 2016
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Cryptosporidium
Detection
Dissemination
Giardia
LAMP
Wastewater

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Gallas-Lindemann, C., Sotiriadou, I., Plutzer, J., Noack, M. J., Mahmoudi, M. R., & Karanis, P. (2016). Giardia and Cryptosporidium spp. dissemination during wastewater treatment and comparative detection via immunofluorescence assay (IFA), nested polymerase chain reaction (nested PCR) and loop mediated isothermal amplification (LAMP). Acta Tropica, 158, 43-51. https://doi.org/10.1016/j.actatropica.2016.02.005

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title = "Giardia and Cryptosporidium spp. dissemination during wastewater treatment and comparative detection via immunofluorescence assay (IFA), nested polymerase chain reaction (nested PCR) and loop mediated isothermal amplification (LAMP)",

abstract = "Environmental water samples from the Lower Rhine area in Germany were investigated via immunofluorescence assays (IFAs), nested polymerase chain reaction (nested PCR) and loop-mediated isothermal amplification (LAMP) to detect the presence of Giardia spp. (n = 185) and Cryptosporidium spp. (n = 227). The samples were concentrated through filtration or flocculation, and oocysts were purified via centrifugation through a sucrose density gradient. For all samples, IFA was performed first, followed by DNA extraction for the nested PCR and LAMP assays. Giardia cysts were detected in 105 samples (56.8\%) by IFA, 62 samples (33.5\%) by nested PCR and 79 samples (42.7\%) by LAMP. Cryptosporidium spp. were detected in 69 samples (30.4\%) by IFA, 95 samples (41.9\%) by nested PCR and 99 samples (43.6\%) by LAMP. According to these results, the three detection methods are complementary for monitoring Giardia and Cryptosporidium in environmental waters.",

keywords = "Cryptosporidium, Detection, Dissemination, Giardia, LAMP, Wastewater",

author = "Carmen Gallas-Lindemann and Isaia Sotiriadou and Judit Plutzer and Noack, \{Michael J.\} and Mahmoudi, \{Mohammad Reza\} and Panagiotis Karanis",

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year = "2016",

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doi = "10.1016/j.actatropica.2016.02.005",

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Gallas-Lindemann, C, Sotiriadou, I, Plutzer, J, Noack, MJ, Mahmoudi, MR & Karanis, P 2016, 'Giardia and Cryptosporidium spp. dissemination during wastewater treatment and comparative detection via immunofluorescence assay (IFA), nested polymerase chain reaction (nested PCR) and loop mediated isothermal amplification (LAMP)', Acta Tropica, vol. 158, pp. 43-51. https://doi.org/10.1016/j.actatropica.2016.02.005

Giardia and Cryptosporidium spp. dissemination during wastewater treatment and comparative detection via immunofluorescence assay (IFA), nested polymerase chain reaction (nested PCR) and loop mediated isothermal amplification (LAMP). / Gallas-Lindemann, Carmen; Sotiriadou, Isaia; Plutzer, Judit et al.
In: Acta Tropica, Vol. 158, 01.06.2016, p. 43-51.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Giardia and Cryptosporidium spp. dissemination during wastewater treatment and comparative detection via immunofluorescence assay (IFA), nested polymerase chain reaction (nested PCR) and loop mediated isothermal amplification (LAMP)

AU - Gallas-Lindemann, Carmen

AU - Sotiriadou, Isaia

AU - Plutzer, Judit

AU - Noack, Michael J.

AU - Mahmoudi, Mohammad Reza

AU - Karanis, Panagiotis

PY - 2016/6/1

Y1 - 2016/6/1

N2 - Environmental water samples from the Lower Rhine area in Germany were investigated via immunofluorescence assays (IFAs), nested polymerase chain reaction (nested PCR) and loop-mediated isothermal amplification (LAMP) to detect the presence of Giardia spp. (n = 185) and Cryptosporidium spp. (n = 227). The samples were concentrated through filtration or flocculation, and oocysts were purified via centrifugation through a sucrose density gradient. For all samples, IFA was performed first, followed by DNA extraction for the nested PCR and LAMP assays. Giardia cysts were detected in 105 samples (56.8%) by IFA, 62 samples (33.5%) by nested PCR and 79 samples (42.7%) by LAMP. Cryptosporidium spp. were detected in 69 samples (30.4%) by IFA, 95 samples (41.9%) by nested PCR and 99 samples (43.6%) by LAMP. According to these results, the three detection methods are complementary for monitoring Giardia and Cryptosporidium in environmental waters.

AB - Environmental water samples from the Lower Rhine area in Germany were investigated via immunofluorescence assays (IFAs), nested polymerase chain reaction (nested PCR) and loop-mediated isothermal amplification (LAMP) to detect the presence of Giardia spp. (n = 185) and Cryptosporidium spp. (n = 227). The samples were concentrated through filtration or flocculation, and oocysts were purified via centrifugation through a sucrose density gradient. For all samples, IFA was performed first, followed by DNA extraction for the nested PCR and LAMP assays. Giardia cysts were detected in 105 samples (56.8%) by IFA, 62 samples (33.5%) by nested PCR and 79 samples (42.7%) by LAMP. Cryptosporidium spp. were detected in 69 samples (30.4%) by IFA, 95 samples (41.9%) by nested PCR and 99 samples (43.6%) by LAMP. According to these results, the three detection methods are complementary for monitoring Giardia and Cryptosporidium in environmental waters.

KW - Cryptosporidium

KW - Detection

KW - Dissemination

KW - Giardia

KW - LAMP

KW - Wastewater

UR - https://www.scopus.com/pages/publications/84959340677

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SN - 0001-706X

VL - 158

SP - 43

EP - 51

JO - Acta Tropica

JF - Acta Tropica

ER -

Gallas-Lindemann C, Sotiriadou I, Plutzer J, Noack MJ, Mahmoudi MR, Karanis P. Giardia and Cryptosporidium spp. dissemination during wastewater treatment and comparative detection via immunofluorescence assay (IFA), nested polymerase chain reaction (nested PCR) and loop mediated isothermal amplification (LAMP). Acta Tropica. 2016 Jun 1;158:43-51. doi: 10.1016/j.actatropica.2016.02.005

Giardia and Cryptosporidium spp. dissemination during wastewater treatment and comparative detection via immunofluorescence assay (IFA), nested polymerase chain reaction (nested PCR) and loop mediated isothermal amplification (LAMP)

Abstract

UN SDGs

Keywords

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