Seven new point mutations have been identified from LH β-subunit gene by PCR-mediated SSCP, and sequencing. One mutation was found changing amino acid from Gln102 to Ser102. The remaining six mutations, which did not change the codings, were in complete linkage disequilibrium. SSCP can be used in the diagnosis of LH-related disorders.
|Number of pages||3|
|Journal||Molecular and Cellular Biochemistry|
|Publication status||Published - 1996|
- Luteinizing hormone
- Polymerase chain reaction
- Restriction enzyme analysis
- Single-stranded conformation polymorphism (SSCP)