TY - JOUR
T1 - In vitro antioxidant activity and phenolic content of Cedrus brevifolia bark
AU - Cretu, Elena
AU - Salminen, Juha Pekka
AU - Karonen, Maarit
AU - Miron, Anca
AU - Charalambous, Christiana
AU - Constantinou, Andreas I.
AU - Aprotosoaie, Ana Clara
PY - 2014
Y1 - 2014
N2 - A raw extract and four extractive fractions were obtained from Cedrus brevifolia (Cyprus cedar) bark. They were all studied regarding the phenolic content and profile using spectrophotometry and HPLC-DAD-ESI-MS. The antioxidant activity was investigated using in vitro assays: DPPH and ABTS radicals scavenging and reducing power assays. The ethyl acetate fraction had the highest total phenolic and proanthocyanidin contents; a taxifolin-O-hexoside, catechin, epicatechin and procyanidin oligomers (three dimers, two trimers) were identified in this fraction. The ethyl acetate fraction was found to possess the highest DPPH and ABTS radicals scavenging effects (EC50=13.9 ± 0.3 and 2.3 ± 0.0 μg/mL, respectively) and reducing capacity (EC50=9.1 ± 0.1 μg/mL). Antioxidant effects were highly correlated with total phenolic and proanthocyanidin contents (r=0.89-0.99). These results suggest that Cedrus brevifolia bark is a new source of antioxidants.
AB - A raw extract and four extractive fractions were obtained from Cedrus brevifolia (Cyprus cedar) bark. They were all studied regarding the phenolic content and profile using spectrophotometry and HPLC-DAD-ESI-MS. The antioxidant activity was investigated using in vitro assays: DPPH and ABTS radicals scavenging and reducing power assays. The ethyl acetate fraction had the highest total phenolic and proanthocyanidin contents; a taxifolin-O-hexoside, catechin, epicatechin and procyanidin oligomers (three dimers, two trimers) were identified in this fraction. The ethyl acetate fraction was found to possess the highest DPPH and ABTS radicals scavenging effects (EC50=13.9 ± 0.3 and 2.3 ± 0.0 μg/mL, respectively) and reducing capacity (EC50=9.1 ± 0.1 μg/mL). Antioxidant effects were highly correlated with total phenolic and proanthocyanidin contents (r=0.89-0.99). These results suggest that Cedrus brevifolia bark is a new source of antioxidants.
KW - ABTS
KW - Cedrus brevifolia
KW - DPPH
KW - Free radical scavenging
KW - HPLC-DAD-ESI-MS
KW - Procyanidins
KW - Reducing power
KW - Taxifolin
UR - http://www.scopus.com/inward/record.url?scp=84898782365&partnerID=8YFLogxK
M3 - Article
C2 - 24868861
AN - SCOPUS:84898782365
SN - 1934-578X
VL - 9
SP - 481
EP - 482
JO - Natural Product Communications
JF - Natural Product Communications
IS - 4
ER -