In vitro antioxidant activity and phenolic content of Cedrus brevifolia bark

Elena Cretu, Juha Pekka Salminen, Maarit Karonen, Anca Miron, Christiana Charalambous, Andreas I. Constantinou, Ana Clara Aprotosoaie

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A raw extract and four extractive fractions were obtained from Cedrus brevifolia (Cyprus cedar) bark. They were all studied regarding the phenolic content and profile using spectrophotometry and HPLC-DAD-ESI-MS. The antioxidant activity was investigated using in vitro assays: DPPH and ABTS radicals scavenging and reducing power assays. The ethyl acetate fraction had the highest total phenolic and proanthocyanidin contents; a taxifolin-O-hexoside, catechin, epicatechin and procyanidin oligomers (three dimers, two trimers) were identified in this fraction. The ethyl acetate fraction was found to possess the highest DPPH and ABTS radicals scavenging effects (EC50=13.9 ± 0.3 and 2.3 ± 0.0 μg/mL, respectively) and reducing capacity (EC50=9.1 ± 0.1 μg/mL). Antioxidant effects were highly correlated with total phenolic and proanthocyanidin contents (r=0.89-0.99). These results suggest that Cedrus brevifolia bark is a new source of antioxidants.

Original languageEnglish
Pages (from-to)481-482
Number of pages2
JournalNatural Product Communications
Volume9
Issue number4
Publication statusPublished - 2014

Keywords

  • ABTS
  • Cedrus brevifolia
  • DPPH
  • Free radical scavenging
  • HPLC-DAD-ESI-MS
  • Procyanidins
  • Reducing power
  • Taxifolin

Fingerprint Dive into the research topics of 'In vitro antioxidant activity and phenolic content of Cedrus brevifolia bark'. Together they form a unique fingerprint.

  • Cite this

    Cretu, E., Salminen, J. P., Karonen, M., Miron, A., Charalambous, C., Constantinou, A. I., & Aprotosoaie, A. C. (2014). In vitro antioxidant activity and phenolic content of Cedrus brevifolia bark. Natural Product Communications, 9(4), 481-482.