In vitro antioxidant activity and phenolic content of Cedrus brevifolia bark

Elena Cretu, Juha Pekka Salminen, Maarit Karonen, Anca Miron, Christiana Charalambous, Andreas I. Constantinou, Ana Clara Aprotosoaie

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


A raw extract and four extractive fractions were obtained from Cedrus brevifolia (Cyprus cedar) bark. They were all studied regarding the phenolic content and profile using spectrophotometry and HPLC-DAD-ESI-MS. The antioxidant activity was investigated using in vitro assays: DPPH and ABTS radicals scavenging and reducing power assays. The ethyl acetate fraction had the highest total phenolic and proanthocyanidin contents; a taxifolin-O-hexoside, catechin, epicatechin and procyanidin oligomers (three dimers, two trimers) were identified in this fraction. The ethyl acetate fraction was found to possess the highest DPPH and ABTS radicals scavenging effects (EC50=13.9 ± 0.3 and 2.3 ± 0.0 μg/mL, respectively) and reducing capacity (EC50=9.1 ± 0.1 μg/mL). Antioxidant effects were highly correlated with total phenolic and proanthocyanidin contents (r=0.89-0.99). These results suggest that Cedrus brevifolia bark is a new source of antioxidants.

Original languageEnglish
Pages (from-to)481-482
Number of pages2
JournalNatural Product Communications
Issue number4
Publication statusPublished - 2014


  • ABTS
  • Cedrus brevifolia
  • DPPH
  • Free radical scavenging
  • Procyanidins
  • Reducing power
  • Taxifolin


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