TY - JOUR
T1 - Infrared spectral features of exfoliated cervical cells, cervical adenocarcinoma tissue, and an adenocarcinoma cell line (SiSo)
AU - Neviliappan, Shanmugam
AU - Fang Kan, Lim
AU - Tiang Lee Walter, Tan
AU - Arulkumaran, Sabaratnam
AU - Wong, P. T T
PY - 2002
Y1 - 2002
N2 - Objective. The objective of the present study is to evaluate and compare the infrared spectral features of normal and malignant exfoliated cervical cells, cells from malignant tissue, and the SiSo cell line. Methods. Infrared spectra of cervical adenocarcinoma (CA) tissue, normal and malignant exfoliated cervical cells, and a uterine cervical adenocarcinoma cell line (SiSo) were obtained. Spectral qualities in terms of band intensity ratio and band position, which reflect configurational changes in the functional groups of the above samples, were measured. Results. Spectral bands of CA tissue, exfoliated cells from CA, and the cell line were similar but markedly different from that of exfoliated normal cervical cells. Significant changes in bands at 1025 cm-1 (glycogen), 1080 cm-1 (glycogen and nucleic acids), 1155 cm-1 (C-OH groups of serine, threonine, and tyrosine of cell proteins, and C-O groups of carbohydrates), 1240 cm-1 (PO2 groups of nucleic acids), 1400 cm-1 (methyl group of lipids and proteins), and 1450 cm-1 (methylene group of lipids and proteins) were noted in the CA tissue, exfoliated CA cells, and adenocarcinoma cell line compared with exfoliated normal cells. Marked shifts in band positions from 1080 to 1086 cm-1, 1153 to 1160 cm-1, and 935 to 970 cm-1 in CA tissue, exfoliated CA cells, and the adenocarcinoma cell line were noted. Conclusion. Spectral bands of the adenocarcinoma cell line matched very well with those of cervical CA tissue and exfoliated CA cells in terms of position. In contrast, spectral bands of the SiSo cell line differed greatly from those of normal exfoliated cells.
AB - Objective. The objective of the present study is to evaluate and compare the infrared spectral features of normal and malignant exfoliated cervical cells, cells from malignant tissue, and the SiSo cell line. Methods. Infrared spectra of cervical adenocarcinoma (CA) tissue, normal and malignant exfoliated cervical cells, and a uterine cervical adenocarcinoma cell line (SiSo) were obtained. Spectral qualities in terms of band intensity ratio and band position, which reflect configurational changes in the functional groups of the above samples, were measured. Results. Spectral bands of CA tissue, exfoliated cells from CA, and the cell line were similar but markedly different from that of exfoliated normal cervical cells. Significant changes in bands at 1025 cm-1 (glycogen), 1080 cm-1 (glycogen and nucleic acids), 1155 cm-1 (C-OH groups of serine, threonine, and tyrosine of cell proteins, and C-O groups of carbohydrates), 1240 cm-1 (PO2 groups of nucleic acids), 1400 cm-1 (methyl group of lipids and proteins), and 1450 cm-1 (methylene group of lipids and proteins) were noted in the CA tissue, exfoliated CA cells, and adenocarcinoma cell line compared with exfoliated normal cells. Marked shifts in band positions from 1080 to 1086 cm-1, 1153 to 1160 cm-1, and 935 to 970 cm-1 in CA tissue, exfoliated CA cells, and the adenocarcinoma cell line were noted. Conclusion. Spectral bands of the adenocarcinoma cell line matched very well with those of cervical CA tissue and exfoliated CA cells in terms of position. In contrast, spectral bands of the SiSo cell line differed greatly from those of normal exfoliated cells.
KW - Adenocarcinoma
KW - Exfoliated cervical cells
KW - Infrared spectroscopy
KW - SiSo cell line
UR - http://www.scopus.com/inward/record.url?scp=0036222964&partnerID=8YFLogxK
U2 - 10.1006/gyno.2002.6602
DO - 10.1006/gyno.2002.6602
M3 - Article
C2 - 11925139
AN - SCOPUS:0036222964
SN - 0090-8258
VL - 85
SP - 170
EP - 174
JO - Gynecologic Oncology
JF - Gynecologic Oncology
IS - 1
ER -