Iron chelators induce apoptosis in proliferating cells

D. Hileti, P. Panayiotidis, A. V. Hoffbrand

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Abstract

The iron chelators 1,2-dimethyl-3-hydroxypyrid-4-one (L1) and desferrioxamine (DFO) were found to induce apoptosis of proliferating activated T-lymphocytes and of the promyelocytic cell line HL60, but not of resting peripheral blood lymphocytes or granulocytes. The induction of apoptosis was quantified by propidium iodide staining of apoptotic/dead cells and flow cytometry. In activated T-lymphocytes incubated with the chelators at equivalent iron-binding concentrations (300 μM L1 or 100 μM DFO) for 24 h, L1 caused a 54% increase in cell death and DFO a 57% increase. In HL60 cells L1 caused a 50% increase in cell death and DFO a 40% increase. DNA cytofluorometry of HL60 cells treated with either chelator showed an increase in the percentage of cells with hypodiploid DNA content. Presaturation of the chelators with ferric chloride abrogated these effects. L1 and DFO did not induce apoptosis in resting peripheral blood lymphocytes or granulocytes, even after 48 h of incubation.

Original languageEnglish
Pages (from-to)181-187
Number of pages7
JournalBritish Journal of Haematology
Volume89
Issue number1
Publication statusPublished - 1995

Keywords

  • 1,2-dimethyl-3-hydroxypyrid-4-one (L)
  • apoptosis
  • deferoxamine
  • iron
  • thalassaemia

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    Hileti, D., Panayiotidis, P., & Hoffbrand, A. V. (1995). Iron chelators induce apoptosis in proliferating cells. British Journal of Haematology, 89(1), 181-187.