Isolating hair follicle stem cells and epidermal keratinocytes from dorsal mouse skin

Despina Soteriou, Lana Kostic, Egor Sedov, Yahav Yosefzon, Hermann Steller, Yaron Fuchs

Research output: Contribution to journalArticlepeer-review

Abstract

The hair follicle (HF) is an ideal system for studying the biology and regulation of adult stem cells (SCs). This dynamic mini organ is replenished by distinct pools of SCs, which are located in the permanent portion of the HF, a region known as the bulge. These multipotent bulge SCs were initially identified as slow cycling label retaining cells; however, their isolation has been made feasible after identification of specific cell markers, such as CD34 and keratin 15 (K15). Here, we describe a robust method for isolating bulge SCs and epidermal keratinocytes from mouse HFs utilizing fluorescence activated cell-sorting (FACS) technology. Isolated hair follicle SCs (HFSCs) can be utilized in various in vivo grafting models and are a valuable in vitro model for studying the mechanisms that govern multipotency, quiescence and activation.

Original languageEnglish
Article numbere53931
JournalJournal of Visualized Experiments
Volume2016
Issue number110
DOIs
Publication statusPublished - 1 Apr 2016

Keywords

  • Cell sorting
  • Developmental biology
  • Dorsal skin dissection
  • FACS
  • Hair follicle
  • Issue 110
  • Mouse
  • Stem cells

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