Abstract
The epidermis consists of several distinct compartments including the inter-follicular epidermis (IFE), sweat glands, sebaceous glands (SGs), and the hair follicle (HF). While the IFE and SGs are in a constant state of self-renewal, the HF cycles between phases of growth, destruction, and rest. The hair follicle stem cells (HFSCs) that fuel this perpetual cycle have been well described and are located in a niche termed the bulge. These bulge SCs express markers such as CD34 and Keratin 15 (K15), enabling the isolation of these cells. Here, we describe a powerful method for isolating HFSCs and epidermal progenitors from mouse skin utilizing fluorescence activated cell-sorting (FACS). Upon isolation, cells can be expanded and utilized in various in vivo and in vitro models aimed at studying the function of these unique cells.
Original language | English |
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Journal | Current Protocols in Stem Cell Biology |
Volume | 2017 |
DOIs | |
Publication status | Published - 1 May 2017 |
Keywords
- Cell sorting
- Epidermis
- FACS
- Hair follicle
- Progenitors
- Skin
- Stem cells