Isolation of stem cells and progenitors from mouse epidermis

Lana Kostic, Egor Sedov, Despina Soteriou, Yahav Yosefzon, Yaron Fuchs

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The epidermis consists of several distinct compartments including the inter-follicular epidermis (IFE), sweat glands, sebaceous glands (SGs), and the hair follicle (HF). While the IFE and SGs are in a constant state of self-renewal, the HF cycles between phases of growth, destruction, and rest. The hair follicle stem cells (HFSCs) that fuel this perpetual cycle have been well described and are located in a niche termed the bulge. These bulge SCs express markers such as CD34 and Keratin 15 (K15), enabling the isolation of these cells. Here, we describe a powerful method for isolating HFSCs and epidermal progenitors from mouse skin utilizing fluorescence activated cell-sorting (FACS). Upon isolation, cells can be expanded and utilized in various in vivo and in vitro models aimed at studying the function of these unique cells.

Original languageEnglish
JournalCurrent Protocols in Stem Cell Biology
Volume2017
DOIs
Publication statusPublished - 1 May 2017

Keywords

  • Cell sorting
  • Epidermis
  • FACS
  • Hair follicle
  • Progenitors
  • Skin
  • Stem cells

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