TY - JOUR
T1 - Kidney Organoids as Disease Models
T2 - Strengths, Weaknesses and Perspectives
AU - Romero-Guevara, Ricardo
AU - Ioannides, Adonis
AU - Xinaris, Christodoulos
N1 - Funding Information:
This work was co-funded by the European Regional Development Fund and the Republic of Cyprus through the Research and Innovation Foundation (Project: POSTDOC/0916/0109). CX research is also funded by Euronanomed (an ERA-NET grant; 736/8221).
Publisher Copyright:
© Copyright © 2020 Romero-Guevara, Ioannides and Xinaris.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/11/4
Y1 - 2020/11/4
N2 - Chronic kidney disease is a major global health problem, as it affects 10% of the global population and kills millions of patients every year. It is therefore of the utmost importance to develop models that can help us to understand the pathogenesis of CKD and improve our therapeutic strategies. The discovery of human induced pluripotent stem cells (hiPSCs) and, more recently, the development of methods for the generation of 3D organoids, have opened the way for modeling human kidney development and disease in vitro, and testing new drugs directly on human tissue. In this review we will discuss the most recent advances in the field of kidney organoids for modeling disease, as well as the prospective applications of these models for drug screening. We will also emphasize the impact of CRISPR/cas9 genome engineering on the field, point out the current limitations of the existing organoid technologies, and discuss a set of technical developments that may help to overcome limitations and facilitate the incorporation of these exciting tools into basic biomedical research.
AB - Chronic kidney disease is a major global health problem, as it affects 10% of the global population and kills millions of patients every year. It is therefore of the utmost importance to develop models that can help us to understand the pathogenesis of CKD and improve our therapeutic strategies. The discovery of human induced pluripotent stem cells (hiPSCs) and, more recently, the development of methods for the generation of 3D organoids, have opened the way for modeling human kidney development and disease in vitro, and testing new drugs directly on human tissue. In this review we will discuss the most recent advances in the field of kidney organoids for modeling disease, as well as the prospective applications of these models for drug screening. We will also emphasize the impact of CRISPR/cas9 genome engineering on the field, point out the current limitations of the existing organoid technologies, and discuss a set of technical developments that may help to overcome limitations and facilitate the incorporation of these exciting tools into basic biomedical research.
KW - 3D cell culture
KW - disease modeling
KW - hiPSC
KW - kidney
KW - organoid
KW - stem cells
UR - http://www.scopus.com/inward/record.url?scp=85096229285&partnerID=8YFLogxK
U2 - 10.3389/fphys.2020.563981
DO - 10.3389/fphys.2020.563981
M3 - Review article
AN - SCOPUS:85096229285
SN - 1664-042X
VL - 11
JO - Frontiers in Physiology
JF - Frontiers in Physiology
M1 - 563981
ER -