TY - JOUR
T1 - Mediterranean diet–gene interactions
T2 - A targeted metabolomics study in Greek-Cypriot women
AU - Kakkoura, Maria G.
AU - Sokratous, Kleitos
AU - Demetriou, Christiana A.
AU - Loizidou, Maria A.
AU - Loucaides, Giorgos
AU - Kakouri, Eleni
AU - Hadjisavvas, Andreas
AU - Kyriacou, Kyriacos
PY - 2017/4/1
Y1 - 2017/4/1
N2 - Scope: A high adherence to the Mediterranean diet (MD) was previously associated with a decreased risk of breast cancer (BC) among Greek-Cypriot women. Additionally, particular polymorphisms were shown to modulate this MD-BC association. Herein, we aimed to investigate the effect of polymorphisms–MD interactions on the levels of specific metabolites that could be related to dietary adherence or enzymatic activity, which is itself modulated by polymorphisms. Methods and results: Greek-Cypriot women who were BC controls and had the lowest or the highest MD adherence (vegetables, fruit, legumes, fish) as assessed by principal component analysis (n = 564) were included. Participants were previously genotyped for nine polymorphisms of the one-carbon metabolism, oxidative stress, and xenobiotic metabolism. The serum levels of 14 metabolites that are key players in the aforementioned pathways were measured by UPLC-MS/MS. ANCOVA was used to assess polymorphism–MD interactions on metabolites’ levels within a multivariate linear regression model. Statistically significant interactions between GSTM1 (where GST is glutathione S-transferase) deletion polymorphism and MD on flavin mononucleotide and on 5-methyltetrahydrofolate (5-MTHF) concentrations were observed. The MTHFR rs1801133 interacted significantly with MD on 5-MTHF concentration. Conclusion: Serum levels of flavin mononucleotide and 5-MTHF were shown to be influenced by interactions between GSTM1 deletion or MTHFR (rs1801133) polymorphisms and a dietary pattern, characteristic of MD.
AB - Scope: A high adherence to the Mediterranean diet (MD) was previously associated with a decreased risk of breast cancer (BC) among Greek-Cypriot women. Additionally, particular polymorphisms were shown to modulate this MD-BC association. Herein, we aimed to investigate the effect of polymorphisms–MD interactions on the levels of specific metabolites that could be related to dietary adherence or enzymatic activity, which is itself modulated by polymorphisms. Methods and results: Greek-Cypriot women who were BC controls and had the lowest or the highest MD adherence (vegetables, fruit, legumes, fish) as assessed by principal component analysis (n = 564) were included. Participants were previously genotyped for nine polymorphisms of the one-carbon metabolism, oxidative stress, and xenobiotic metabolism. The serum levels of 14 metabolites that are key players in the aforementioned pathways were measured by UPLC-MS/MS. ANCOVA was used to assess polymorphism–MD interactions on metabolites’ levels within a multivariate linear regression model. Statistically significant interactions between GSTM1 (where GST is glutathione S-transferase) deletion polymorphism and MD on flavin mononucleotide and on 5-methyltetrahydrofolate (5-MTHF) concentrations were observed. The MTHFR rs1801133 interacted significantly with MD on 5-MTHF concentration. Conclusion: Serum levels of flavin mononucleotide and 5-MTHF were shown to be influenced by interactions between GSTM1 deletion or MTHFR (rs1801133) polymorphisms and a dietary pattern, characteristic of MD.
KW - Mediterranean dietary pattern
KW - Nutrigenetics
KW - One-carbon metabolism
KW - Serum-targeted metabolomics
KW - Xenobiotic metabolism
UR - http://www.scopus.com/inward/record.url?scp=85017138754&partnerID=8YFLogxK
U2 - 10.1002/mnfr.201600558
DO - 10.1002/mnfr.201600558
M3 - Article
C2 - 27860207
AN - SCOPUS:85017138754
SN - 1613-4125
VL - 61
JO - Molecular Nutrition and Food Research
JF - Molecular Nutrition and Food Research
IS - 4
M1 - 1600558
ER -