Molecular and immunological characterization of Mus a 5 allergen from banana fruit

Ivana Aleksic, Milica Popovic, Rajna Dimitrijevic, Uros Andjelkovic, Emilia Vassilopoulou, Athanassios Sinaniotis, Marina Atanaskovic-Markovic, Buko Lindner, Arnd Petersen, Nikolaos G. Papadopoulos, Marija Gavrovic-Jankulovic

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)


Scope Banana fruit has become an important cause of fruit allergy in the recent years. Among the five registered IUIS allergens, Mus a 1 and Mus a 2 have been characterized in detail. In this study, molecular characterization and evaluation of the allergenic properties of β-1,3-glucanase from banana (Musa acuminata), denoted as Mus a 5, were performed Methods and results The gene of Mus a 5 was cloned and sequenced. The obtained cDNA revealed a novel Mus a 5 isoform with an open reading frame encoding a protein of 340 amino acids comprising a putative signal peptide of 28 amino acid residues. By MALDI-TOF analysis Mus a 5 isolated from banana fruit revealed a molecular mass of 33451±67Da. Two Mus a 5 isoforms (pI 7.7 and 8.0) were detected by 2D immunoblot with an identical N-terminal sequence. By mass fingerprint, 76 and 83% of the primary structure was confirmed for the two mature Mus a 5 isoforms, respectively. IgE reactivity to Mus a 5 was found in 74% of patients sensitized to banana fruit. Upregulation of basophil activation markers CD63 and CD203c was achieved with Mus a 5 in a concentration-dependent manner Conclusion Mus a 5 is a functional allergen and a candidate for the component-resolved allergy diagnosis of banana allergy.

Original languageEnglish
Pages (from-to)446-453
Number of pages8
JournalMolecular Nutrition and Food Research
Issue number3
Publication statusPublished - Mar 2012


  • Allergen
  • Banana
  • Food allergy
  • Glucanase
  • Mus a 5


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