TY - JOUR
T1 - Morphological methods in the diagnosis of mitochondrial encephalomyopathies
T2 - The role of electron microscopy
AU - Kyriacou, K.
AU - Hadjisavvas, A.
AU - Zenios, A.
AU - Papacharalambous, R.
AU - Kyriakides, Th
PY - 2005/5
Y1 - 2005/5
N2 - Mitochondrial encephalomyopathies (MEs) encompass a heterogeneous group of disorders that frequently present a diagnostic challenge to clinicians. Historically, MEs were diagnosed by finding ragged red fibers in the muscle biopsy and confirmatory evidence was provided by the presence of numerical and/or ultrastructural abnormalities in mitochondria. In most centers diagnosis involves clinical evaluation and the morphological, histochemical, and biochemical investigation of a skeletal muscle biopsy. However, with the availability of mitochondrial DNA analysis, the necessity and role of morphological methods and, in particular, electron microscopy has been questioned. The aim of this study was to delineate the role of electron microscopy in the diagnosis of MEs.
AB - Mitochondrial encephalomyopathies (MEs) encompass a heterogeneous group of disorders that frequently present a diagnostic challenge to clinicians. Historically, MEs were diagnosed by finding ragged red fibers in the muscle biopsy and confirmatory evidence was provided by the presence of numerical and/or ultrastructural abnormalities in mitochondria. In most centers diagnosis involves clinical evaluation and the morphological, histochemical, and biochemical investigation of a skeletal muscle biopsy. However, with the availability of mitochondrial DNA analysis, the necessity and role of morphological methods and, in particular, electron microscopy has been questioned. The aim of this study was to delineate the role of electron microscopy in the diagnosis of MEs.
KW - Electron microscopy
KW - Histochemistry
KW - Histology
KW - Mitochondrial encephalomyopathies
UR - http://www.scopus.com/inward/record.url?scp=22244446483&partnerID=8YFLogxK
U2 - 10.1080/01913120590951158
DO - 10.1080/01913120590951158
M3 - Article
C2 - 16036873
AN - SCOPUS:22244446483
SN - 0191-3123
VL - 29
SP - 169
EP - 174
JO - Ultrastructural Pathology
JF - Ultrastructural Pathology
IS - 3-4
ER -