MuSK autoantibodies in myasthenia gravis detected by cell based assay - A multinational study

A. I. Tsonis; P. Zisimopoulou; K. Lazaridis; J. Tzartos; E. Matsigkou; V. Zouvelou; R. Mantegazza; C. Antozzi; F. Andreetta; A. Evoli; F. Deymeer; G. Saruhan-Direskeneli; H. Durmus; T. Brenner; A. Vaknin; S. Berrih-Aknin; A. Behin; T. Sharshar; M. De Baets; M. Losen; P. Martinez-Martinez; K. A. Kleopa; E. Zamba-Papanicolaou; T. Kyriakides; A. Kostera-Pruszczyk; P. Szczudlik; B. Szyluk; D. Lavrnic; I. Basta; S. Peric; C. Tallaksen; A. Maniaol; C. Casasnovas Pons; J. Pitha; M. Jakubíkova; F. Hanisch; S. J. Tzartos

doi:10.1016/j.jneuroim.2015.04.015

MuSK autoantibodies in myasthenia gravis detected by cell based assay - A multinational study

A. I. Tsonis
, P. Zisimopoulou
, K. Lazaridis
, J. Tzartos
, E. Matsigkou
, V. Zouvelou
, R. Mantegazza
, C. Antozzi
, F. Andreetta
, A. Evoli
, F. Deymeer
, G. Saruhan-Direskeneli
, H. Durmus
, T. Brenner
, A. Vaknin
, S. Berrih-Aknin
, A. Behin
, T. Sharshar
, M. De Baets
, M. Losen

P. Martinez-Martinez, K. A. Kleopa, E. Zamba-Papanicolaou, T. Kyriakides, A. Kostera-Pruszczyk, P. Szczudlik, B. Szyluk, D. Lavrnic, I. Basta, S. Peric, C. Tallaksen, A. Maniaol, C. Casasnovas Pons, J. Pitha, M. Jakubíkova, F. Hanisch, S. J. Tzartos

Medical School

Research output: Contribution to journal › Article › peer-review

Abstract

Seronegative myasthenia gravis (MG) presents a serious gap in MG diagnosis and understanding. We applied a cell based assay (CBA) for the detection of muscle specific kinase (MuSK) antibodies undetectable by radioimmunoassay. We tested 633 triple-seronegative MG patients' sera from 13 countries, detecting 13% as positive. MuSK antibodies were found, at significantly lower frequencies, in 1.9% of healthy controls and 5.1% of other neuroimmune disease patients, including multiple sclerosis and neuromyelitis optica. The clinical data of the newly diagnosed MuSK-MG patients are presented. 27% of ocular seronegative patients were MuSK antibody positive. Moreover, 23% had thymic hyperplasia suggesting that thymic abnormalities are more common than believed.

Original language	English
Pages (from-to)	10-17
Number of pages	8
Journal	Journal of Neuroimmunology
Volume	284
DOIs	https://doi.org/10.1016/j.jneuroim.2015.04.015
Publication status	Published - 15 Jul 2015

Keywords

Autoantibodies
Cell based assay
Diagnosis
MuSK
Myasthenia gravis

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10.1016/j.jneuroim.2015.04.015

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Tsonis, A. I., Zisimopoulou, P., Lazaridis, K., Tzartos, J., Matsigkou, E., Zouvelou, V., Mantegazza, R., Antozzi, C., Andreetta, F., Evoli, A., Deymeer, F., Saruhan-Direskeneli, G., Durmus, H., Brenner, T., Vaknin, A., Berrih-Aknin, S., Behin, A., Sharshar, T., De Baets, M., ... Tzartos, S. J. (2015). MuSK autoantibodies in myasthenia gravis detected by cell based assay - A multinational study. Journal of Neuroimmunology, 284, 10-17. https://doi.org/10.1016/j.jneuroim.2015.04.015

@article{8041435fc93b4134b44f1334e80624e1,

title = "MuSK autoantibodies in myasthenia gravis detected by cell based assay - A multinational study",

abstract = "Seronegative myasthenia gravis (MG) presents a serious gap in MG diagnosis and understanding. We applied a cell based assay (CBA) for the detection of muscle specific kinase (MuSK) antibodies undetectable by radioimmunoassay. We tested 633 triple-seronegative MG patients' sera from 13 countries, detecting 13\% as positive. MuSK antibodies were found, at significantly lower frequencies, in 1.9\% of healthy controls and 5.1\% of other neuroimmune disease patients, including multiple sclerosis and neuromyelitis optica. The clinical data of the newly diagnosed MuSK-MG patients are presented. 27\% of ocular seronegative patients were MuSK antibody positive. Moreover, 23\% had thymic hyperplasia suggesting that thymic abnormalities are more common than believed.",

keywords = "Autoantibodies, Cell based assay, Diagnosis, MuSK, Myasthenia gravis",

author = "Tsonis, \{A. I.\} and P. Zisimopoulou and K. Lazaridis and J. Tzartos and E. Matsigkou and V. Zouvelou and R. Mantegazza and C. Antozzi and F. Andreetta and A. Evoli and F. Deymeer and G. Saruhan-Direskeneli and H. Durmus and T. Brenner and A. Vaknin and S. Berrih-Aknin and A. Behin and T. Sharshar and \{De Baets\}, M. and M. Losen and P. Martinez-Martinez and Kleopa, \{K. A.\} and E. Zamba-Papanicolaou and T. Kyriakides and A. Kostera-Pruszczyk and P. Szczudlik and B. Szyluk and D. Lavrnic and I. Basta and S. Peric and C. Tallaksen and A. Maniaol and \{Casasnovas Pons\}, C. and J. Pitha and M. Jakub{\'i}kova and F. Hanisch and Tzartos, \{S. J.\}",

year = "2015",

month = jul,

day = "15",

doi = "10.1016/j.jneuroim.2015.04.015",

language = "English",

volume = "284",

pages = "10--17",

journal = "Journal of Neuroimmunology",

issn = "0165-5728",

publisher = "Elsevier B.V.",

}

Tsonis, AI, Zisimopoulou, P, Lazaridis, K, Tzartos, J, Matsigkou, E, Zouvelou, V, Mantegazza, R, Antozzi, C, Andreetta, F, Evoli, A, Deymeer, F, Saruhan-Direskeneli, G, Durmus, H, Brenner, T, Vaknin, A, Berrih-Aknin, S, Behin, A, Sharshar, T, De Baets, M, Losen, M, Martinez-Martinez, P, Kleopa, KA, Zamba-Papanicolaou, E, Kyriakides, T, Kostera-Pruszczyk, A, Szczudlik, P, Szyluk, B, Lavrnic, D, Basta, I, Peric, S, Tallaksen, C, Maniaol, A, Casasnovas Pons, C, Pitha, J, Jakubíkova, M, Hanisch, F & Tzartos, SJ 2015, 'MuSK autoantibodies in myasthenia gravis detected by cell based assay - A multinational study', Journal of Neuroimmunology, vol. 284, pp. 10-17. https://doi.org/10.1016/j.jneuroim.2015.04.015

TY - JOUR

T1 - MuSK autoantibodies in myasthenia gravis detected by cell based assay - A multinational study

AU - Tsonis, A. I.

AU - Zisimopoulou, P.

AU - Lazaridis, K.

AU - Tzartos, J.

AU - Matsigkou, E.

AU - Zouvelou, V.

AU - Mantegazza, R.

AU - Antozzi, C.

AU - Andreetta, F.

AU - Evoli, A.

AU - Deymeer, F.

AU - Saruhan-Direskeneli, G.

AU - Durmus, H.

AU - Brenner, T.

AU - Vaknin, A.

AU - Berrih-Aknin, S.

AU - Behin, A.

AU - Sharshar, T.

AU - De Baets, M.

AU - Losen, M.

AU - Martinez-Martinez, P.

AU - Kleopa, K. A.

AU - Zamba-Papanicolaou, E.

AU - Kyriakides, T.

AU - Kostera-Pruszczyk, A.

AU - Szczudlik, P.

AU - Szyluk, B.

AU - Lavrnic, D.

AU - Basta, I.

AU - Peric, S.

AU - Tallaksen, C.

AU - Maniaol, A.

AU - Casasnovas Pons, C.

AU - Pitha, J.

AU - Jakubíkova, M.

AU - Hanisch, F.

AU - Tzartos, S. J.

PY - 2015/7/15

Y1 - 2015/7/15

N2 - Seronegative myasthenia gravis (MG) presents a serious gap in MG diagnosis and understanding. We applied a cell based assay (CBA) for the detection of muscle specific kinase (MuSK) antibodies undetectable by radioimmunoassay. We tested 633 triple-seronegative MG patients' sera from 13 countries, detecting 13% as positive. MuSK antibodies were found, at significantly lower frequencies, in 1.9% of healthy controls and 5.1% of other neuroimmune disease patients, including multiple sclerosis and neuromyelitis optica. The clinical data of the newly diagnosed MuSK-MG patients are presented. 27% of ocular seronegative patients were MuSK antibody positive. Moreover, 23% had thymic hyperplasia suggesting that thymic abnormalities are more common than believed.

AB - Seronegative myasthenia gravis (MG) presents a serious gap in MG diagnosis and understanding. We applied a cell based assay (CBA) for the detection of muscle specific kinase (MuSK) antibodies undetectable by radioimmunoassay. We tested 633 triple-seronegative MG patients' sera from 13 countries, detecting 13% as positive. MuSK antibodies were found, at significantly lower frequencies, in 1.9% of healthy controls and 5.1% of other neuroimmune disease patients, including multiple sclerosis and neuromyelitis optica. The clinical data of the newly diagnosed MuSK-MG patients are presented. 27% of ocular seronegative patients were MuSK antibody positive. Moreover, 23% had thymic hyperplasia suggesting that thymic abnormalities are more common than believed.

KW - Autoantibodies

KW - Cell based assay

KW - Diagnosis

KW - MuSK

KW - Myasthenia gravis

UR - https://www.scopus.com/pages/publications/84929849327

U2 - 10.1016/j.jneuroim.2015.04.015

DO - 10.1016/j.jneuroim.2015.04.015

M3 - Article

C2 - 26025053

AN - SCOPUS:84929849327

SN - 0165-5728

VL - 284

SP - 10

EP - 17

JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

ER -

MuSK autoantibodies in myasthenia gravis detected by cell based assay - A multinational study

Abstract

Keywords

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