MUTATION PREVENTING FORMATION OF HEPATITIS B e ANTIGEN IN PATIENTS WITH CHRONIC HEPATITIS B INFECTION

W. F. Carman, S. Hadziyannis, M. J. Mcgarvey, M. R. Jacyna, P. Karayiannis, A. Makris, H. C. Thomas

Research output: Contribution to journalArticle

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Abstract

Some patients with chronic hepatitis B virus (HBV) infection are HB e antigen (HBeAg) negative, have circulating HBV particles, and often have especially severe chronic hepatitis. To test the hypothesis that the absence of HBeAg production may be due to a change in the nucleotide sequence of the pre-core region of the genome, 18 Greek and 3 non-Greek patients positive for HB surface antigen underwent direct sequencing of HBV-DNA amplified from sera. In 7 out of 8 HBeAg negative patients, two mutations (guanosine to adenosine) were found in the terminal two codons of the pre-core region, giving the sequence TAGGACATG. The remaining patient had the first mutation only. The sequence TGGGGCATG was found in 4 of 5 of the HBeAg positive patients. The first mutation results in a translational stop codon that is predicted to result in failure to produce HBeAg. The rest of the pre-core region in the HBeAg negative patients was otherwise homologous to that of the HBeAg positive patients and to known sequences.

Original languageEnglish
Pages (from-to)588-591
Number of pages4
JournalThe Lancet
Volume334
Issue number8663
DOIs
Publication statusPublished - 9 Sep 1989

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Hepatitis B e Antigens
Chronic Hepatitis B
Mutation
Infection
Hepatitis B virus
Terminator Codon
Guanosine
Virus Diseases
Chronic Hepatitis
Surface Antigens
Codon
Virion
Adenosine
Genome
DNA
Serum

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Carman, W. F., Hadziyannis, S., Mcgarvey, M. J., Jacyna, M. R., Karayiannis, P., Makris, A., & Thomas, H. C. (1989). MUTATION PREVENTING FORMATION OF HEPATITIS B e ANTIGEN IN PATIENTS WITH CHRONIC HEPATITIS B INFECTION. The Lancet, 334(8663), 588-591. https://doi.org/10.1016/S0140-6736(89)90713-7
Carman, W. F. ; Hadziyannis, S. ; Mcgarvey, M. J. ; Jacyna, M. R. ; Karayiannis, P. ; Makris, A. ; Thomas, H. C. / MUTATION PREVENTING FORMATION OF HEPATITIS B e ANTIGEN IN PATIENTS WITH CHRONIC HEPATITIS B INFECTION. In: The Lancet. 1989 ; Vol. 334, No. 8663. pp. 588-591.
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Carman, WF, Hadziyannis, S, Mcgarvey, MJ, Jacyna, MR, Karayiannis, P, Makris, A & Thomas, HC 1989, 'MUTATION PREVENTING FORMATION OF HEPATITIS B e ANTIGEN IN PATIENTS WITH CHRONIC HEPATITIS B INFECTION', The Lancet, vol. 334, no. 8663, pp. 588-591. https://doi.org/10.1016/S0140-6736(89)90713-7

MUTATION PREVENTING FORMATION OF HEPATITIS B e ANTIGEN IN PATIENTS WITH CHRONIC HEPATITIS B INFECTION. / Carman, W. F.; Hadziyannis, S.; Mcgarvey, M. J.; Jacyna, M. R.; Karayiannis, P.; Makris, A.; Thomas, H. C.

In: The Lancet, Vol. 334, No. 8663, 09.09.1989, p. 588-591.

Research output: Contribution to journalArticle

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