MUTATION PREVENTING FORMATION OF HEPATITIS B e ANTIGEN IN PATIENTS WITH CHRONIC HEPATITIS B INFECTION

W. F. Carman, S. Hadziyannis, M. J. Mcgarvey, M. R. Jacyna, P. Karayiannis, A. Makris, H. C. Thomas

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Abstract

Some patients with chronic hepatitis B virus (HBV) infection are HB e antigen (HBeAg) negative, have circulating HBV particles, and often have especially severe chronic hepatitis. To test the hypothesis that the absence of HBeAg production may be due to a change in the nucleotide sequence of the pre-core region of the genome, 18 Greek and 3 non-Greek patients positive for HB surface antigen underwent direct sequencing of HBV-DNA amplified from sera. In 7 out of 8 HBeAg negative patients, two mutations (guanosine to adenosine) were found in the terminal two codons of the pre-core region, giving the sequence TAGGACATG. The remaining patient had the first mutation only. The sequence TGGGGCATG was found in 4 of 5 of the HBeAg positive patients. The first mutation results in a translational stop codon that is predicted to result in failure to produce HBeAg. The rest of the pre-core region in the HBeAg negative patients was otherwise homologous to that of the HBeAg positive patients and to known sequences.

Original languageEnglish
Pages (from-to)588-591
Number of pages4
JournalThe Lancet
Volume334
Issue number8663
DOIs
Publication statusPublished - 9 Sep 1989

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    Carman, W. F., Hadziyannis, S., Mcgarvey, M. J., Jacyna, M. R., Karayiannis, P., Makris, A., & Thomas, H. C. (1989). MUTATION PREVENTING FORMATION OF HEPATITIS B e ANTIGEN IN PATIENTS WITH CHRONIC HEPATITIS B INFECTION. The Lancet, 334(8663), 588-591. https://doi.org/10.1016/S0140-6736(89)90713-7