TY - JOUR
T1 - Occurrence of Cryptosporidium and Giardia in wild birds from Qinghai Lake on the Qinghai-Tibetan Plateau, China
AU - Jian, Yingna
AU - Zhang, Xueyong
AU - Li, Xiuping
AU - Schou, Chad
AU - Charalambidou, Iris
AU - Ma, Liqing
AU - Karanis, Panagiotis
N1 - Funding Information:
We are grateful to the staff Yubang He, Yonglin Wu, and Yuansheng Hou working in Qinghai Lake National Nature Reserve Administration for their assistance with collecting the samples.
Funding Information:
The research was supported by the National Natural Science Foundation of China (no. 32060804), the Young and Middle-Aged Research Funding Project from Qinghai University (2017-QNY-1) and the One Thousand Talents Plan (P. Karanis) of the Chinese Government (no. WQ2013630172).
Publisher Copyright:
© 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.
PY - 2021/2
Y1 - 2021/2
N2 - Cryptosporidium and Giardia are important intestinal zoonotic pathogens that can infect various hosts and cause diarrhoeal diseases. There are few reports of the epidemiological prevalence and molecular characterization of Cryptosporidium and Giardia in wild birds around Qinghai Lake and in the surrounding areas on the Qinghai-Tibetan Plateau, Northwest China. Therefore, the aim of this study was to determine the Cryptosporidium spp. and Giardia duodenalis genotypes and their epidemiological prevalence in wild birds by PCR amplification. To our knowledge, this is the first report of a variety of Cryptosporidium spp. and G. duodenalis infections in wild birds from that area, with overall prevalence rates of 8.98% (61/679) and 3.39% (23/679), respectively. Furthermore, PCR sequencing confirmed the presence of Cryptosporidium baileyi (n = 3), Cryptosporidium parvum (n = 58), and G. duodenalis assemblage B (n = 19) and E (n = 4) in wild birds from the areas around Qinghai Lake. The results of the present study demonstrated the wide distribution of Cryptosporidium and Giardia among wild birds, which has potential public health significance. Moreover, the study findings also provided useful molecular epidemiological data for monitoring and investigating the two parasitic protozoa in wild animals and surrounding environments.
AB - Cryptosporidium and Giardia are important intestinal zoonotic pathogens that can infect various hosts and cause diarrhoeal diseases. There are few reports of the epidemiological prevalence and molecular characterization of Cryptosporidium and Giardia in wild birds around Qinghai Lake and in the surrounding areas on the Qinghai-Tibetan Plateau, Northwest China. Therefore, the aim of this study was to determine the Cryptosporidium spp. and Giardia duodenalis genotypes and their epidemiological prevalence in wild birds by PCR amplification. To our knowledge, this is the first report of a variety of Cryptosporidium spp. and G. duodenalis infections in wild birds from that area, with overall prevalence rates of 8.98% (61/679) and 3.39% (23/679), respectively. Furthermore, PCR sequencing confirmed the presence of Cryptosporidium baileyi (n = 3), Cryptosporidium parvum (n = 58), and G. duodenalis assemblage B (n = 19) and E (n = 4) in wild birds from the areas around Qinghai Lake. The results of the present study demonstrated the wide distribution of Cryptosporidium and Giardia among wild birds, which has potential public health significance. Moreover, the study findings also provided useful molecular epidemiological data for monitoring and investigating the two parasitic protozoa in wild animals and surrounding environments.
KW - Cryptosporidium
KW - Giardia
KW - Molecular characterization
KW - Prevalence
KW - Qinghai Lake (China)
KW - Wild birds
UR - http://www.scopus.com/inward/record.url?scp=85099112988&partnerID=8YFLogxK
U2 - 10.1007/s00436-020-06993-w
DO - 10.1007/s00436-020-06993-w
M3 - Article
C2 - 33415392
AN - SCOPUS:85099112988
SN - 0932-0113
VL - 120
SP - 615
EP - 628
JO - Parasitology Research
JF - Parasitology Research
IS - 2
ER -
