Opioid Receptors in Magnesium‐Digitonin‐Solubilized Rat Brain Membranes Are Tightly Coupled to a Pertussis Toxin‐Sensitive Guanine Nucleotide‐Binding Protein

Yung H. Wong, Catherine D. Demoliou‐Mason, Eric A. Barnard

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Abstract Opioid receptors solubilized in Mg2+‐digitonin (2%, wt/vol) from Mg2+‐pretreated rat brain membranes maintain, in addition to high‐affinity opioid agonist binding, the modulation by guanine nucleotides. One of the modes of expression of the latter property is an attenuation of agonist binding by guanine nucleotides in the presence of Na+. To investigate the molecular basis of this modulation and to identify the G protein(s) involved, the soluble receptors were [32P]ADP‐ribosylated by means of Bordetella pertussis toxin and subjected to molecular size exclusion chromatography. In addition, soluble extracts were chromatographed on lectin and hydrophobic affinity columns. The binding of 35S‐ and 3H‐labelled analogues of GTP was also monitored in the species separated. The oligomeric G protein‐coupled opioid receptors and the guanine nucleotide/pertussis toxin‐sensitive species showed similar chromatographic properties in all three systems. This indicates that the biochemically functional G protein‐opioid receptor complex formed in Mg2+‐pretreated membranes in the absence of an agonist is stable in digitonin solution and to chromatographic separation. Further analysis showed that the guanine nucleotide modulation of opioid receptors is via the pertussis toxin substrates with Mr of 41,000 and 39,000, which are identified as Gi and Goα subunits, respectively.

Original languageEnglish
Pages (from-to)999-1009
Number of pages11
JournalJournal of Neurochemistry
Volume52
Issue number4
DOIs
Publication statusPublished - 1989

Fingerprint

Guanine Nucleotides
Whooping Cough
Guanine
Opioid Receptors
Rats
Brain
Membranes
Digitonin
Pertussis Toxin
Modulation
Proteins
Bordetella pertussis
Size exclusion chromatography
Guanosine Triphosphate
GTP-Binding Proteins
Lectins
Opioid Analgesics
Gel Chromatography
Substrates

Keywords

  • G proteins
  • Opioid receptors
  • Pertussis toxin

Cite this

@article{5c0ca9a051e24931827386ec886b865e,
title = "Opioid Receptors in Magnesium‐Digitonin‐Solubilized Rat Brain Membranes Are Tightly Coupled to a Pertussis Toxin‐Sensitive Guanine Nucleotide‐Binding Protein",
abstract = "Abstract Opioid receptors solubilized in Mg2+‐digitonin (2{\%}, wt/vol) from Mg2+‐pretreated rat brain membranes maintain, in addition to high‐affinity opioid agonist binding, the modulation by guanine nucleotides. One of the modes of expression of the latter property is an attenuation of agonist binding by guanine nucleotides in the presence of Na+. To investigate the molecular basis of this modulation and to identify the G protein(s) involved, the soluble receptors were [32P]ADP‐ribosylated by means of Bordetella pertussis toxin and subjected to molecular size exclusion chromatography. In addition, soluble extracts were chromatographed on lectin and hydrophobic affinity columns. The binding of 35S‐ and 3H‐labelled analogues of GTP was also monitored in the species separated. The oligomeric G protein‐coupled opioid receptors and the guanine nucleotide/pertussis toxin‐sensitive species showed similar chromatographic properties in all three systems. This indicates that the biochemically functional G protein‐opioid receptor complex formed in Mg2+‐pretreated membranes in the absence of an agonist is stable in digitonin solution and to chromatographic separation. Further analysis showed that the guanine nucleotide modulation of opioid receptors is via the pertussis toxin substrates with Mr of 41,000 and 39,000, which are identified as Gi and Goα subunits, respectively.",
keywords = "G proteins, Opioid receptors, Pertussis toxin",
author = "Wong, {Yung H.} and Demoliou‐Mason, {Catherine D.} and Barnard, {Eric A.}",
year = "1989",
doi = "10.1111/j.1471-4159.1989.tb01840.x",
language = "English",
volume = "52",
pages = "999--1009",
journal = "Journal of Neurochemistry",
issn = "0022-3042",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - Opioid Receptors in Magnesium‐Digitonin‐Solubilized Rat Brain Membranes Are Tightly Coupled to a Pertussis Toxin‐Sensitive Guanine Nucleotide‐Binding Protein

AU - Wong, Yung H.

AU - Demoliou‐Mason, Catherine D.

AU - Barnard, Eric A.

PY - 1989

Y1 - 1989

N2 - Abstract Opioid receptors solubilized in Mg2+‐digitonin (2%, wt/vol) from Mg2+‐pretreated rat brain membranes maintain, in addition to high‐affinity opioid agonist binding, the modulation by guanine nucleotides. One of the modes of expression of the latter property is an attenuation of agonist binding by guanine nucleotides in the presence of Na+. To investigate the molecular basis of this modulation and to identify the G protein(s) involved, the soluble receptors were [32P]ADP‐ribosylated by means of Bordetella pertussis toxin and subjected to molecular size exclusion chromatography. In addition, soluble extracts were chromatographed on lectin and hydrophobic affinity columns. The binding of 35S‐ and 3H‐labelled analogues of GTP was also monitored in the species separated. The oligomeric G protein‐coupled opioid receptors and the guanine nucleotide/pertussis toxin‐sensitive species showed similar chromatographic properties in all three systems. This indicates that the biochemically functional G protein‐opioid receptor complex formed in Mg2+‐pretreated membranes in the absence of an agonist is stable in digitonin solution and to chromatographic separation. Further analysis showed that the guanine nucleotide modulation of opioid receptors is via the pertussis toxin substrates with Mr of 41,000 and 39,000, which are identified as Gi and Goα subunits, respectively.

AB - Abstract Opioid receptors solubilized in Mg2+‐digitonin (2%, wt/vol) from Mg2+‐pretreated rat brain membranes maintain, in addition to high‐affinity opioid agonist binding, the modulation by guanine nucleotides. One of the modes of expression of the latter property is an attenuation of agonist binding by guanine nucleotides in the presence of Na+. To investigate the molecular basis of this modulation and to identify the G protein(s) involved, the soluble receptors were [32P]ADP‐ribosylated by means of Bordetella pertussis toxin and subjected to molecular size exclusion chromatography. In addition, soluble extracts were chromatographed on lectin and hydrophobic affinity columns. The binding of 35S‐ and 3H‐labelled analogues of GTP was also monitored in the species separated. The oligomeric G protein‐coupled opioid receptors and the guanine nucleotide/pertussis toxin‐sensitive species showed similar chromatographic properties in all three systems. This indicates that the biochemically functional G protein‐opioid receptor complex formed in Mg2+‐pretreated membranes in the absence of an agonist is stable in digitonin solution and to chromatographic separation. Further analysis showed that the guanine nucleotide modulation of opioid receptors is via the pertussis toxin substrates with Mr of 41,000 and 39,000, which are identified as Gi and Goα subunits, respectively.

KW - G proteins

KW - Opioid receptors

KW - Pertussis toxin

UR - http://www.scopus.com/inward/record.url?scp=0024583845&partnerID=8YFLogxK

U2 - 10.1111/j.1471-4159.1989.tb01840.x

DO - 10.1111/j.1471-4159.1989.tb01840.x

M3 - Article

C2 - 2538569

AN - SCOPUS:0024583845

VL - 52

SP - 999

EP - 1009

JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

SN - 0022-3042

IS - 4

ER -