Peptide synthesis: Methods, trends, and challenges

    Research output: Chapter in Book/Report/Conference proceedingChapter

    Abstract

    This chapter provides an overview of peptide synthesis giving emphasis on solid-phase peptide synthesis (SPPS). Currently, most of the peptides used for research or therapeutics are synthesized by SPPS methods. Successful peptide synthesis overcoming the usual handles and limitations is extremely dependent on the convenient selection of resins, linkers, amino acid derivatives, and coupling reagents as well as side chain (de)protection and cleavage conditions. Old and newer solid supports, linkers, protecting groups, coupling reagents, deprotection, and/or cleavage solutions widely used in Fmoc/. tBu methodology are presented and discussed. Newer greener ways of synthesis are also presented. The chapter concludes with future challenges, perspectives and opportunities in the field of solid-phase peptide chemistry.

    Original languageEnglish
    Title of host publicationPeptide Applications in Biomedicine, Biotechnology and Bioengineering
    PublisherElsevier Inc.
    Pages1-21
    Number of pages21
    ISBN (Electronic)9780081007426
    ISBN (Print)9780081007365
    DOIs
    Publication statusPublished - 16 Nov 2017

    Keywords

    • Cleavage solution
    • Coupling reagents
    • Cyclization reagents
    • Disulfide formation
    • Fmoc/tBu methodology
    • Linkers
    • Solid-phase peptide synthesis
    • SPPS

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  • Cite this

    Petrou, C., & Sarigiannis, Y. (2017). Peptide synthesis: Methods, trends, and challenges. In Peptide Applications in Biomedicine, Biotechnology and Bioengineering (pp. 1-21). Elsevier Inc.. https://doi.org/10.1016/B978-0-08-100736-5.00001-6