TY - JOUR
T1 - Peptidomimetic-functionalized carbon nanotubes with antitrypsin activity
AU - Karousis, Nikolaos
AU - Papi, Rigini M.
AU - Siskos, Argiris
AU - Vakalopoulou, Paraskevi
AU - Glezakos, Petros
AU - Sarigiannis, Yiannis
AU - Stavropoulos, Georgios
AU - Kyriakidis, Dimitrios A.
AU - Tagmatarchis, Nikos
PY - 2009/12
Y1 - 2009/12
N2 - Water-soluble functionalized carbon nanotubes (CNTs) have been prepared and further conjugated through a stable covalent bond with two peptidomimetics. The structural design of the covalently grafted peptidomimetics to the CNTs is based on their structural similarity with the metabolites of antagonist G of substance P. A variety of analytical spectroscopic methods, in combination with electron microscopy and thermal analysis, aided the structural and morphological characterization of the four newly synthesized peptidomimetic-CNT conjugates. It is demonstrated that the trypsin inhibitory effect of the peptidomimetic-CNT is enhanced as a result of the high-loading of peptidomimetics onto the skeleton of the modified water-soluble CNTs. Additionally, the peptidomimetic-functionalized CNT conjugates can be recovered and re-employed up to six biological evaluation cycles showing the same trypsin inhibitory activity. Such a nanosized system is extremely advantageous for the inhibition of inflammation or malignancy and could find potential future biological applications in the area of drug delivery systems.
AB - Water-soluble functionalized carbon nanotubes (CNTs) have been prepared and further conjugated through a stable covalent bond with two peptidomimetics. The structural design of the covalently grafted peptidomimetics to the CNTs is based on their structural similarity with the metabolites of antagonist G of substance P. A variety of analytical spectroscopic methods, in combination with electron microscopy and thermal analysis, aided the structural and morphological characterization of the four newly synthesized peptidomimetic-CNT conjugates. It is demonstrated that the trypsin inhibitory effect of the peptidomimetic-CNT is enhanced as a result of the high-loading of peptidomimetics onto the skeleton of the modified water-soluble CNTs. Additionally, the peptidomimetic-functionalized CNT conjugates can be recovered and re-employed up to six biological evaluation cycles showing the same trypsin inhibitory activity. Such a nanosized system is extremely advantageous for the inhibition of inflammation or malignancy and could find potential future biological applications in the area of drug delivery systems.
UR - http://www.scopus.com/inward/record.url?scp=70349141736&partnerID=8YFLogxK
U2 - 10.1016/j.carbon.2009.08.025
DO - 10.1016/j.carbon.2009.08.025
M3 - Article
AN - SCOPUS:70349141736
SN - 0008-6223
VL - 47
SP - 3550
EP - 3558
JO - Carbon
JF - Carbon
IS - 15
ER -