Polypeptide Amino Acid Composition and Isoelectric Point II. Comparison between Experiment and Theory

C. S. Patrickios, E. N. Yamasaki

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Experimental isoelectric points and amino acid compositional data for 58 proteins were compiled and organized. The experimental isoelectric points correlated well with the acidic to basic amino acid molar ratio. This agreement proved the usefulness of a recently presented analytical expression correlating explicitly protein isoelectric point to acid-base composition. Regressed acidic and basic dissociation constants were determined to be pKa = 4.9 and pKb = 10.0, in fair agreement with the expected values of pKa = 4.2 and pKb = 11.2. Theoretical isoelectric points determined by a more complete computational procedure were on the average in as good an agreement with the experimental values as those calculated via the theoretical approximation using the regressed dissociation constants. Thus, the analytical approximation is a powerful tool for the convenient and accurate calculation of protein isoelectric point from the amino acid composition.

Original languageEnglish
Pages (from-to)82-91
Number of pages10
JournalAnalytical Biochemistry
Issue number1
Publication statusPublished - Oct 1995

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