Abstract
Site-directed spin labeling electron paramagnetic resonance methods have been an important tool in studying protein-protein interactions. Labels are often attached to a cysteine residue, and spectra are acquired with and without binding partner(s) to provide information on the binding. This requires a knowledge of the label location which is simplified if the label remains faithfully attached to the designated residue in the complex. We report a system where this is not the case because the label was extracted by dialysis-resistant glutathione molecules. Once this artifact is identified, spectral subtraction provides a solution for meaningful data interpretation.
Original language | English |
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Pages (from-to) | 160-162 |
Number of pages | 3 |
Journal | Analytical Biochemistry |
Volume | 376 |
Issue number | 1 |
DOIs | |
Publication status | Published - 1 May 2008 |