Potential artifacts in using a glutathione S-transferase fusion protein system and spin labeling electron paramagnetic resonance methods to study protein-protein interactions

Chloe Antoniou, L. W M Fung

Research output: Contribution to journalArticlepeer-review

Abstract

Site-directed spin labeling electron paramagnetic resonance methods have been an important tool in studying protein-protein interactions. Labels are often attached to a cysteine residue, and spectra are acquired with and without binding partner(s) to provide information on the binding. This requires a knowledge of the label location which is simplified if the label remains faithfully attached to the designated residue in the complex. We report a system where this is not the case because the label was extracted by dialysis-resistant glutathione molecules. Once this artifact is identified, spectral subtraction provides a solution for meaningful data interpretation.

Original languageEnglish
Pages (from-to)160-162
Number of pages3
JournalAnalytical Biochemistry
Volume376
Issue number1
DOIs
Publication statusPublished - 1 May 2008

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