Predictive Molecular Biomarkers of Bladder Cancer Identified by Next-Generation Sequencing—Preliminary Data

Aleksander Myszka, Marek Ciesla, Aleksandra Siekierzynska, Anna Sendera, Constantina Constantinou, Pawel Karpinski, Grzegorz Wysiadecki, Krzysztof Balawender

Research output: Contribution to journalArticlepeer-review

Abstract

Background: The majority of patients with bladder cancer suffer from tumour recurrence. Identifying prognostic factors for tumour recurrence is crucial for treatment and follow-up in affected patients. The study aimed to assess the impact of somatic mutations in bladder cancer on patient outcomes and tumour recurrence. Methods: The study group comprised 46 patients with urothelial bladder cancers referred for transurethral resection of the tumour. A molecular study on tumour-derived DNA was performed using next-generation sequencing. Somatic mutations were screened in 50 genes involved in carcinogenesis. Results: We identified 81 variants in 23 genes, including 54 pathogenic mutations, 18 likely pathogenic variants, and 9 variants of unknown significance. The most frequently mutated genes were FGFR3, PIK3CA, and TP53 in 52%, 35%, and 24% of tumours, respectively. The average tumour-free survival was significantly longer in cases with mutations in the PIK3CA gene (p = 0.02), and mutations in the PIK3CA gene were associated with a decreased risk of tumour recurrence (Hazard Ratio = 0.26; 95% CI: 0.11–0.62; p = 0.018). Conclusions: The PIK3CA gene was shown to be a predictive marker of a low risk of bladder tumour recurrence. Molecular screening of bladder cancers supported predictive biomarkers of tumour recurrence and showed that tumour-free survival is molecularly determined.

Original languageEnglish
Article number7701
JournalJournal of Clinical Medicine
Volume13
Issue number24
DOIs
Publication statusPublished - Dec 2024
Externally publishedYes

Keywords

  • bladder cancer recurrence
  • disease-free survival
  • molecular biomarkers
  • somatic mutations

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