Replicative competence of the T131I, K141E, and G145R surface variants of hepatitis B virus

Saffie Jammeh, Howard C. Thomas, Peter Karayiannis

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Variants of hepatitis B surface antigen have been described in different clinical settings, but their replicative capacity in vitro has remained unexplored. Point mutations leading to sT131I, sK141E, and sG145R amino-acid substitutions were engineered by site-directed mutagenesis into an infectious plasmid clone of the virus. The mutated constructs were transfected into Huh7 cells, and their replication capacity was documented by LightCycler (Roche Diagnostics) measurements of virion-associated hepatitis B virus (HBV) DNA, intracellular relaxed circular double-stranded DNA, and pregenomic RNA. The sT131I and sG145R variants replicated with efficiency equal to that of the wild type, whereas the sK141E variant was replication impaired.

Original languageEnglish
Pages (from-to)1010-1013
Number of pages4
JournalJournal of Infectious Diseases
Volume196
Issue number7
DOIs
Publication statusPublished - 2007

Fingerprint Dive into the research topics of 'Replicative competence of the T131I, K141E, and G145R surface variants of hepatitis B virus'. Together they form a unique fingerprint.

  • Cite this