Abstract
The binding sites for opiates (agonist and antagonist) and opioid peptides can be solubilized from rat brain membranes with digitonin in the presence of Mg2+ (10 mM). High affinity and high capacity binding to the soluble δ, μ, and κ receptors is obtainable when the membranes are treated in Mg2+ (30°C, 60 min) prior to solubilization. The yields of solubilized binding sites extracted with digitonin, 40-90%, are higher than those obtained from Mg2+-pretreated membranes with other detergents commonly used for receptor solubilization. The stability of the digitonin-soluble opioid receptor at room temperature makes it useful for purification and characterization.
Original language | English |
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Pages (from-to) | 378-382 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 170 |
Issue number | 2 |
DOIs | |
Publication status | Published - 21 May 1984 |
Keywords
- Opioid receptor
- Receptor solubilization
- Subtype