Solubilization in high yield of opioid receptors retaining high-affinity delta, mu and kappa binding sites

Research output: Contribution to journalArticlepeer-review

Abstract

The binding sites for opiates (agonist and antagonist) and opioid peptides can be solubilized from rat brain membranes with digitonin in the presence of Mg2+ (10 mM). High affinity and high capacity binding to the soluble δ, μ, and κ receptors is obtainable when the membranes are treated in Mg2+ (30°C, 60 min) prior to solubilization. The yields of solubilized binding sites extracted with digitonin, 40-90%, are higher than those obtained from Mg2+-pretreated membranes with other detergents commonly used for receptor solubilization. The stability of the digitonin-soluble opioid receptor at room temperature makes it useful for purification and characterization.

Original languageEnglish
Pages (from-to)378-382
Number of pages5
JournalFEBS Letters
Volume170
Issue number2
DOIs
Publication statusPublished - 21 May 1984

Keywords

  • Opioid receptor
  • Receptor solubilization
  • Subtype

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