SV40-mediated immortalization of human fibroblasts

Harvey L. Ozer; Satnam S. Banga; Tanya Dasgupta; Jeanmarie Houghton; Karen Hubbard; Krishna K. Jha; Soo Hyun Kim; Melanie Lenahan; Zeng Pang; Jose R. Pardinas; Philippos C. Patsalis

doi:10.1016/0531-5565(95)00024-0

SV40-mediated immortalization of human fibroblasts

Harvey L. Ozer
, Satnam S. Banga
, Tanya Dasgupta
, Jeanmarie Houghton
, Karen Hubbard
, Krishna K. Jha
, Soo Hyun Kim
, Melanie Lenahan
, Zeng Pang
, Jose R. Pardinas
, Philippos C. Patsalis

Research output: Contribution to journal › Article › peer-review

Abstract

We have identified a multistep mechanism by which the DNA virus SV40 overcomes cellular senescence. Expression of SV40 T antigen is required for both transient extension of life span and unlimited life span or immortalization. These effects are mediated through inactivation of function of growth suppressors pRB and p53 via complex formation with T antigen. However, immortalization additionally requires inactivation of a novel growth suppressor gene, which has recently been identified to be on the distal portion of the long arm of chromosome 6, designated SEN6. We propose that SEN6 is responsible for cellular senescence in fibroblasts and other cells.

Original language	English
Pages (from-to)	303-310
Number of pages	8
Journal	Experimental Gerontology
Volume	31
Issue number	1-2
DOIs	https://doi.org/10.1016/0531-5565(95)00024-0
Publication status	Published - 1996
Externally published	Yes

Keywords

chromosome 6
growth suppressor
immortalization
p53
RB
SEN6
senescence
SV40
T antigen
transformed human fibroblasts

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10.1016/0531-5565(95)00024-0

Cite this

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title = "SV40-mediated immortalization of human fibroblasts",

abstract = "We have identified a multistep mechanism by which the DNA virus SV40 overcomes cellular senescence. Expression of SV40 T antigen is required for both transient extension of life span and unlimited life span or immortalization. These effects are mediated through inactivation of function of growth suppressors pRB and p53 via complex formation with T antigen. However, immortalization additionally requires inactivation of a novel growth suppressor gene, which has recently been identified to be on the distal portion of the long arm of chromosome 6, designated SEN6. We propose that SEN6 is responsible for cellular senescence in fibroblasts and other cells.",

keywords = "chromosome 6, growth suppressor, immortalization, p53, RB, SEN6, senescence, SV40, T antigen, transformed human fibroblasts",

author = "Ozer, \{Harvey L.\} and Banga, \{Satnam S.\} and Tanya Dasgupta and Jeanmarie Houghton and Karen Hubbard and Jha, \{Krishna K.\} and Kim, \{Soo Hyun\} and Melanie Lenahan and Zeng Pang and Pardinas, \{Jose R.\} and Patsalis, \{Philippos C.\}",

year = "1996",

doi = "10.1016/0531-5565(95)00024-0",

language = "English",

volume = "31",

pages = "303--310",

journal = "Experimental Gerontology",

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number = "1-2",

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TY - JOUR

T1 - SV40-mediated immortalization of human fibroblasts

AU - Ozer, Harvey L.

AU - Banga, Satnam S.

AU - Dasgupta, Tanya

AU - Houghton, Jeanmarie

AU - Hubbard, Karen

AU - Jha, Krishna K.

AU - Kim, Soo Hyun

AU - Lenahan, Melanie

AU - Pang, Zeng

AU - Pardinas, Jose R.

AU - Patsalis, Philippos C.

PY - 1996

Y1 - 1996

N2 - We have identified a multistep mechanism by which the DNA virus SV40 overcomes cellular senescence. Expression of SV40 T antigen is required for both transient extension of life span and unlimited life span or immortalization. These effects are mediated through inactivation of function of growth suppressors pRB and p53 via complex formation with T antigen. However, immortalization additionally requires inactivation of a novel growth suppressor gene, which has recently been identified to be on the distal portion of the long arm of chromosome 6, designated SEN6. We propose that SEN6 is responsible for cellular senescence in fibroblasts and other cells.

AB - We have identified a multistep mechanism by which the DNA virus SV40 overcomes cellular senescence. Expression of SV40 T antigen is required for both transient extension of life span and unlimited life span or immortalization. These effects are mediated through inactivation of function of growth suppressors pRB and p53 via complex formation with T antigen. However, immortalization additionally requires inactivation of a novel growth suppressor gene, which has recently been identified to be on the distal portion of the long arm of chromosome 6, designated SEN6. We propose that SEN6 is responsible for cellular senescence in fibroblasts and other cells.

KW - chromosome 6

KW - growth suppressor

KW - immortalization

KW - p53

KW - RB

KW - SEN6

KW - senescence

KW - SV40

KW - T antigen

KW - transformed human fibroblasts

UR - https://www.scopus.com/pages/publications/0029874219

U2 - 10.1016/0531-5565(95)00024-0

DO - 10.1016/0531-5565(95)00024-0

M3 - Article

C2 - 8706800

AN - SCOPUS:0029874219

SN - 0531-5565

VL - 31

SP - 303

EP - 310

JO - Experimental Gerontology

JF - Experimental Gerontology

IS - 1-2

ER -

SV40-mediated immortalization of human fibroblasts

Abstract

Keywords

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