Temporal and spatial expression of TGF-β1 in an Achilles tendon section model after application of platelet-rich plasma

Dimitrios N. Lyras, Konstantinos Kazakos, Marios Tryfonidis, George Agrogiannis, Sotirios Botaitis, Anna Kokka, George Drosos, Konstantinos Tilkeridis, Dionysios Verettas

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27 Citations (Scopus)

Abstract

Background: To investigate the effect of platelet-rich plasma (PRP) on TGF-β1 expression during tendon healing. Methods: We used 48 skeletally mature New Zealand White rabbits. 24 rabbits received the PRP, and 24 rabbits served as an untreated control group. Equal numbers of animals were sacrificed at 1st, 2nd, 3rd, and 4th week. The surgical procedure involved a transverse incision to transect the Achilles tendon. A volume of 1. ml of PRP was then injected into the tendon mass in the PRP group. Histological and immunohistochemical evaluations with an anti-TGF-β primary antibody were performed. Results: The pattern of expression of TGF-β1 in the PRP group was characterized by a significant upregulation during the first 2 weeks and subsequently significant downregulation in the 3rd and 4th week in comparison with the controls. Conclusions: Our results suggest that PRP may affect the tendon healing process by altering the expression of TGF-β1.

Original languageEnglish
Pages (from-to)137-141
Number of pages5
JournalFoot and Ankle Surgery
Volume16
Issue number3
DOIs
Publication statusPublished - Sep 2010

Keywords

  • Immunohistochemistry
  • Platelet-rich plasma
  • Rabbits
  • Tendon healing
  • TGF-β1

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    Lyras, D. N., Kazakos, K., Tryfonidis, M., Agrogiannis, G., Botaitis, S., Kokka, A., Drosos, G., Tilkeridis, K., & Verettas, D. (2010). Temporal and spatial expression of TGF-β1 in an Achilles tendon section model after application of platelet-rich plasma. Foot and Ankle Surgery, 16(3), 137-141. https://doi.org/10.1016/j.fas.2009.09.002