TY - JOUR
T1 - Temporal and spatial expression of TGF-β1 in an Achilles tendon section model after application of platelet-rich plasma
AU - Lyras, Dimitrios N.
AU - Kazakos, Konstantinos
AU - Tryfonidis, Marios
AU - Agrogiannis, George
AU - Botaitis, Sotirios
AU - Kokka, Anna
AU - Drosos, George
AU - Tilkeridis, Konstantinos
AU - Verettas, Dionysios
PY - 2010/9
Y1 - 2010/9
N2 - Background: To investigate the effect of platelet-rich plasma (PRP) on TGF-β1 expression during tendon healing. Methods: We used 48 skeletally mature New Zealand White rabbits. 24 rabbits received the PRP, and 24 rabbits served as an untreated control group. Equal numbers of animals were sacrificed at 1st, 2nd, 3rd, and 4th week. The surgical procedure involved a transverse incision to transect the Achilles tendon. A volume of 1. ml of PRP was then injected into the tendon mass in the PRP group. Histological and immunohistochemical evaluations with an anti-TGF-β primary antibody were performed. Results: The pattern of expression of TGF-β1 in the PRP group was characterized by a significant upregulation during the first 2 weeks and subsequently significant downregulation in the 3rd and 4th week in comparison with the controls. Conclusions: Our results suggest that PRP may affect the tendon healing process by altering the expression of TGF-β1.
AB - Background: To investigate the effect of platelet-rich plasma (PRP) on TGF-β1 expression during tendon healing. Methods: We used 48 skeletally mature New Zealand White rabbits. 24 rabbits received the PRP, and 24 rabbits served as an untreated control group. Equal numbers of animals were sacrificed at 1st, 2nd, 3rd, and 4th week. The surgical procedure involved a transverse incision to transect the Achilles tendon. A volume of 1. ml of PRP was then injected into the tendon mass in the PRP group. Histological and immunohistochemical evaluations with an anti-TGF-β primary antibody were performed. Results: The pattern of expression of TGF-β1 in the PRP group was characterized by a significant upregulation during the first 2 weeks and subsequently significant downregulation in the 3rd and 4th week in comparison with the controls. Conclusions: Our results suggest that PRP may affect the tendon healing process by altering the expression of TGF-β1.
KW - Immunohistochemistry
KW - Platelet-rich plasma
KW - Rabbits
KW - Tendon healing
KW - TGF-β1
UR - http://www.scopus.com/inward/record.url?scp=77955056713&partnerID=8YFLogxK
U2 - 10.1016/j.fas.2009.09.002
DO - 10.1016/j.fas.2009.09.002
M3 - Article
C2 - 20655014
AN - SCOPUS:77955056713
SN - 1268-7731
VL - 16
SP - 137
EP - 141
JO - Foot and Ankle Surgery
JF - Foot and Ankle Surgery
IS - 3
ER -