The advantage of a build-up anti-globulin technique for the detection of immunoglobulin on the red cells of rabbits infected with trypanosomes. a preliminary report

B. E. Dodd, G. C. Jenkins, P. J. Lincoln, P. McCrorie

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Abstract

A build-up anti-globulin technique has been used to detect immunoglobulin on the red cells of rabbits infected with Trypanosoma brucei brucei S 42 and 427. The method, which is useful for the detection of immunoglobulin on sparsely coated red cells, involves treating the red cells of infected rabbits with antiglobulin followed by alternate globulin and antiglobulin layers until a lattice is built up which allows agglutination to occur. The red cells of five infected rabbits have been tested with anti-rabbit IgG, -IgM and broad spectrum anti-globulin reagents at intervals after infection and following the administration of Berenil, a drug which destroys trypanosomes. The positive anti-globulin reactions which developed following inoculation with trypanosomes were much reduced after giving Berenil. The tests became more strongly positive again in two rabbits after reinfection.

Original languageEnglish
Pages (from-to)501-505
Number of pages5
JournalTransactions of the Royal Society of Tropical Medicine and Hygiene
Volume72
Issue number5
DOIs
Publication statusPublished - 1978

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Trypanosomiasis
Globulins
Immunoglobulins
Rabbits
Anti-Idiotypic Antibodies
Trypanosoma brucei brucei
Agglutination
Immunoglobulin M
Infection
Pharmaceutical Preparations

Cite this

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abstract = "A build-up anti-globulin technique has been used to detect immunoglobulin on the red cells of rabbits infected with Trypanosoma brucei brucei S 42 and 427. The method, which is useful for the detection of immunoglobulin on sparsely coated red cells, involves treating the red cells of infected rabbits with antiglobulin followed by alternate globulin and antiglobulin layers until a lattice is built up which allows agglutination to occur. The red cells of five infected rabbits have been tested with anti-rabbit IgG, -IgM and broad spectrum anti-globulin reagents at intervals after infection and following the administration of Berenil, a drug which destroys trypanosomes. The positive anti-globulin reactions which developed following inoculation with trypanosomes were much reduced after giving Berenil. The tests became more strongly positive again in two rabbits after reinfection.",
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AU - Lincoln, P. J.

AU - McCrorie, P.

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