The role of calf serum in the growth of Chlamydia trachomatis in McCoy cell cultures

P. Karayiannis, D. Hobson

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Chlamydia trachomatis is normally grown in McCoy monolayer coverslip cultures using partially defined media containing foetal calf serum at concentrations up to 10% (v/v). Omission of the serum decreased the number of inclusions produced by infecting the McCoy cells with a standard inoculum of a genital strain of C. trachomatis. Substitution of the foetal calf serum with a macromolecular fraction from the serum or with a mixture of sodium oleate, bovine serum albumin fraction V and fetuin maintained the inclusion count, but substitution with serum filtrate, containing the amino acids and other low molecular weight components, decreased the inclusion count of C. trachomatis. The role of calf serum and the need for a fully defined medium excluding serum for studying the growth of C. trachomatis in tissue culture are discussed.

Original languageEnglish
Pages (from-to)47-54
Number of pages8
JournalJournal of General Microbiology
Volume122
Issue number1
Publication statusPublished - 1981

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Chlamydia trachomatis
Cell Culture Techniques
Growth
Serum
Fetuins
Bovine Serum Albumin
Molecular Weight
Amino Acids

Cite this

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The role of calf serum in the growth of Chlamydia trachomatis in McCoy cell cultures. / Karayiannis, P.; Hobson, D.

In: Journal of General Microbiology, Vol. 122, No. 1, 1981, p. 47-54.

Research output: Contribution to journalArticle

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AU - Hobson, D.

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