Transcription of rDNA is essential for satellite association

K. A. McDowell; M. Hadjiargyrou; P. Patsalis; Ann S. Henderson

doi:10.1159/000133666

Transcription of rDNA is essential for satellite association

K. A. McDowell
, M. Hadjiargyrou
, P. Patsalis
, Ann S. Henderson

Research output: Contribution to journal › Article › peer-review

Abstract

A nonacrocentric human chromosome containing transfected human ribosomal DNA was analyzed for participation in nonrandom satellite associations with acrocentric chromosomes. Two constructs were tested. The first contained the complete rDNA transcriptional unit plus flanking DNA and was inserted at chromosome 3p13→p12 following transfection. The region was silver-positive indicating active transcription. The transcriptionally active rDNA participated in satellite association with other rDNA-bearing chromosomes at a rate 15% above random background associations. The second construct lacked the promoter region. This rDNA integrated at chromosome 4q31→q32, and was silver stain-negative. Non- random associations with other rDNA-bearing chromosomes were not observed. The study demonstrates that the DNA critical to nonrandom satellite associations is rDNA perse and that active transcription is a requirement.

Original language	English
Pages (from-to)	63-67
Number of pages	5
Journal	Cytogenetics and Cell Genetics
Volume	66
Issue number	1
DOIs	https://doi.org/10.1159/000133666
Publication status	Published - 1994
Externally published	Yes

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title = "Transcription of rDNA is essential for satellite association",

abstract = "A nonacrocentric human chromosome containing transfected human ribosomal DNA was analyzed for participation in nonrandom satellite associations with acrocentric chromosomes. Two constructs were tested. The first contained the complete rDNA transcriptional unit plus flanking DNA and was inserted at chromosome 3p13→p12 following transfection. The region was silver-positive indicating active transcription. The transcriptionally active rDNA participated in satellite association with other rDNA-bearing chromosomes at a rate 15\% above random background associations. The second construct lacked the promoter region. This rDNA integrated at chromosome 4q31→q32, and was silver stain-negative. Non- random associations with other rDNA-bearing chromosomes were not observed. The study demonstrates that the DNA critical to nonrandom satellite associations is rDNA perse and that active transcription is a requirement.",

author = "McDowell, \{K. A.\} and M. Hadjiargyrou and P. Patsalis and Henderson, \{Ann S.\}",

year = "1994",

doi = "10.1159/000133666",

language = "English",

volume = "66",

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journal = "Cytogenetics and Cell Genetics",

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T1 - Transcription of rDNA is essential for satellite association

AU - McDowell, K. A.

AU - Hadjiargyrou, M.

AU - Patsalis, P.

AU - Henderson, Ann S.

PY - 1994

Y1 - 1994

N2 - A nonacrocentric human chromosome containing transfected human ribosomal DNA was analyzed for participation in nonrandom satellite associations with acrocentric chromosomes. Two constructs were tested. The first contained the complete rDNA transcriptional unit plus flanking DNA and was inserted at chromosome 3p13→p12 following transfection. The region was silver-positive indicating active transcription. The transcriptionally active rDNA participated in satellite association with other rDNA-bearing chromosomes at a rate 15% above random background associations. The second construct lacked the promoter region. This rDNA integrated at chromosome 4q31→q32, and was silver stain-negative. Non- random associations with other rDNA-bearing chromosomes were not observed. The study demonstrates that the DNA critical to nonrandom satellite associations is rDNA perse and that active transcription is a requirement.

AB - A nonacrocentric human chromosome containing transfected human ribosomal DNA was analyzed for participation in nonrandom satellite associations with acrocentric chromosomes. Two constructs were tested. The first contained the complete rDNA transcriptional unit plus flanking DNA and was inserted at chromosome 3p13→p12 following transfection. The region was silver-positive indicating active transcription. The transcriptionally active rDNA participated in satellite association with other rDNA-bearing chromosomes at a rate 15% above random background associations. The second construct lacked the promoter region. This rDNA integrated at chromosome 4q31→q32, and was silver stain-negative. Non- random associations with other rDNA-bearing chromosomes were not observed. The study demonstrates that the DNA critical to nonrandom satellite associations is rDNA perse and that active transcription is a requirement.

UR - https://www.scopus.com/pages/publications/0028014335

U2 - 10.1159/000133666

DO - 10.1159/000133666

M3 - Article

C2 - 8275712

AN - SCOPUS:0028014335

SN - 0301-0171

VL - 66

SP - 63

EP - 67

JO - Cytogenetics and Cell Genetics

JF - Cytogenetics and Cell Genetics

IS - 1

ER -

Transcription of rDNA is essential for satellite association

Abstract

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