Transport of 14C-deferiprone in normal, thalassaemic and sickle red blood cells

Oded Shalev; Dona Hileti; Philip Nortey; Robert P. Hebbel; Victor A. Hoffbrand

doi:10.1046/j.1365-2141.1999.01447.x

Transport of 14C-deferiprone in normal, thalassaemic and sickle red blood cells

Oded Shalev, Dona Hileti, Philip Nortey, Robert P. Hebbel, Victor A. Hoffbrand

Research output: Contribution to journal › Article › peer-review

Abstract

The transport of deferiprone (L1) in normal (N), sickle (S) and thalassaemic (T) red blood cells (RBC) was determined by incubation with ¹⁴C-L1 at 37°C. Following incubation with 0.5 mM ¹⁴C-L1 for 4h, the intracellular concentration of L1 in T RBC was 3 times higher than was found extracellularly. In contrast, no concentration gradient across N and S RBC membranes was detected. Efflux studies showed that T RBC released only 17 ± 2% of ¹⁴C-L1 into the extracellular space. We hypothesize that L1 accumulation in T RBC results from their high content of chelatable iron and formation of large, hydrophilic L1-Fe(III) complexes trapped within the cytosol.

Original language	English
Pages (from-to)	1081-1083
Number of pages	3
Journal	British Journal of Haematology
Volume	105
Issue number	4
DOIs	https://doi.org/10.1046/j.1365-2141.1999.01447.x
Publication status	Published - 1999

Keywords

Deferiprone
Erythrocytes
Sickle cell disease
Thalassaemia
Transport

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10.1046/j.1365-2141.1999.01447.x

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title = "Transport of 14C-deferiprone in normal, thalassaemic and sickle red blood cells",

abstract = "The transport of deferiprone (L1) in normal (N), sickle (S) and thalassaemic (T) red blood cells (RBC) was determined by incubation with 14C-L1 at 37°C. Following incubation with 0.5 mM 14C-L1 for 4h, the intracellular concentration of L1 in T RBC was 3 times higher than was found extracellularly. In contrast, no concentration gradient across N and S RBC membranes was detected. Efflux studies showed that T RBC released only 17 ± 2% of 14C-L1 into the extracellular space. We hypothesize that L1 accumulation in T RBC results from their high content of chelatable iron and formation of large, hydrophilic L1-Fe(III) complexes trapped within the cytosol.",

keywords = "Deferiprone, Erythrocytes, Sickle cell disease, Thalassaemia, Transport",

author = "Oded Shalev and Dona Hileti and Philip Nortey and Hebbel, {Robert P.} and Hoffbrand, {Victor A.}",

year = "1999",

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TY - JOUR

T1 - Transport of 14C-deferiprone in normal, thalassaemic and sickle red blood cells

AU - Shalev, Oded

AU - Hileti, Dona

AU - Nortey, Philip

AU - Hebbel, Robert P.

AU - Hoffbrand, Victor A.

PY - 1999

Y1 - 1999

N2 - The transport of deferiprone (L1) in normal (N), sickle (S) and thalassaemic (T) red blood cells (RBC) was determined by incubation with 14C-L1 at 37°C. Following incubation with 0.5 mM 14C-L1 for 4h, the intracellular concentration of L1 in T RBC was 3 times higher than was found extracellularly. In contrast, no concentration gradient across N and S RBC membranes was detected. Efflux studies showed that T RBC released only 17 ± 2% of 14C-L1 into the extracellular space. We hypothesize that L1 accumulation in T RBC results from their high content of chelatable iron and formation of large, hydrophilic L1-Fe(III) complexes trapped within the cytosol.

AB - The transport of deferiprone (L1) in normal (N), sickle (S) and thalassaemic (T) red blood cells (RBC) was determined by incubation with 14C-L1 at 37°C. Following incubation with 0.5 mM 14C-L1 for 4h, the intracellular concentration of L1 in T RBC was 3 times higher than was found extracellularly. In contrast, no concentration gradient across N and S RBC membranes was detected. Efflux studies showed that T RBC released only 17 ± 2% of 14C-L1 into the extracellular space. We hypothesize that L1 accumulation in T RBC results from their high content of chelatable iron and formation of large, hydrophilic L1-Fe(III) complexes trapped within the cytosol.

KW - Deferiprone

KW - Erythrocytes

KW - Sickle cell disease

KW - Thalassaemia

KW - Transport

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U2 - 10.1046/j.1365-2141.1999.01447.x

DO - 10.1046/j.1365-2141.1999.01447.x

M3 - Article

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AN - SCOPUS:0032589580

SN - 0007-1048

VL - 105

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JO - British Journal of Haematology

JF - British Journal of Haematology

IS - 4

ER -

Transport of 14C-deferiprone in normal, thalassaemic and sickle red blood cells

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Keywords

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