Use of biotinylated probes in serum hepatitis B virus DNA detection

J. A. Saldanha; P. Karayiannis; H. C. Thomas; J. P. Monjardino

doi:10.1016/0166-0934(87)90019-X

Use of biotinylated probes in serum hepatitis B virus DNA detection

J. A. Saldanha, P. Karayiannis, H. C. Thomas, J. P. Monjardino

Medical School

University College London

Research output: Contribution to journal › Article › peer-review

Abstract

A dot-blot hybridisation assay for serum HBV DNA is described using a non-radioactive (biotinylated) DNA probe. The assay is both sensitive (1 pg of HBV DNA) and reproducible, and shows several advantages over similar assays which use ³²P-labelled probes for the routine detection of infectious virus particles both in blood and in other biological fluids.

Original language	English
Pages (from-to)	339-342
Number of pages	4
Journal	Journal of Virological Methods
Volume	16
Issue number	4
DOIs	https://doi.org/10.1016/0166-0934(87)90019-X
Publication status	Published - 1987

Keywords

Biotin
DNA
HBV
Hybridisation
Serum

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0166-0934(87)90019-X

Cite this

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title = "Use of biotinylated probes in serum hepatitis B virus DNA detection",

abstract = "A dot-blot hybridisation assay for serum HBV DNA is described using a non-radioactive (biotinylated) DNA probe. The assay is both sensitive (1 pg of HBV DNA) and reproducible, and shows several advantages over similar assays which use 32P-labelled probes for the routine detection of infectious virus particles both in blood and in other biological fluids.",

keywords = "Biotin, DNA, HBV, Hybridisation, Serum",

author = "Saldanha, {J. A.} and P. Karayiannis and Thomas, {H. C.} and Monjardino, {J. P.}",

year = "1987",

doi = "10.1016/0166-0934(87)90019-X",

language = "English",

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pages = "339--342",

journal = "Journal of Virological Methods",

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TY - JOUR

T1 - Use of biotinylated probes in serum hepatitis B virus DNA detection

AU - Saldanha, J. A.

AU - Karayiannis, P.

AU - Thomas, H. C.

AU - Monjardino, J. P.

PY - 1987

Y1 - 1987

N2 - A dot-blot hybridisation assay for serum HBV DNA is described using a non-radioactive (biotinylated) DNA probe. The assay is both sensitive (1 pg of HBV DNA) and reproducible, and shows several advantages over similar assays which use 32P-labelled probes for the routine detection of infectious virus particles both in blood and in other biological fluids.

AB - A dot-blot hybridisation assay for serum HBV DNA is described using a non-radioactive (biotinylated) DNA probe. The assay is both sensitive (1 pg of HBV DNA) and reproducible, and shows several advantages over similar assays which use 32P-labelled probes for the routine detection of infectious virus particles both in blood and in other biological fluids.

KW - Biotin

KW - DNA

KW - HBV

KW - Hybridisation

KW - Serum

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U2 - 10.1016/0166-0934(87)90019-X

DO - 10.1016/0166-0934(87)90019-X

M3 - Article

C2 - 3667852

AN - SCOPUS:0023226744

SN - 0166-0934

VL - 16

SP - 339

EP - 342

JO - Journal of Virological Methods

JF - Journal of Virological Methods

IS - 4

ER -

Use of biotinylated probes in serum hepatitis B virus DNA detection

Abstract

Keywords

UN SDGs

Access to Document

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