Use of biotinylated probes in serum hepatitis B virus DNA detection

J. A. Saldanha, P. Karayiannis, H. C. Thomas, J. P. Monjardino

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A dot-blot hybridisation assay for serum HBV DNA is described using a non-radioactive (biotinylated) DNA probe. The assay is both sensitive (1 pg of HBV DNA) and reproducible, and shows several advantages over similar assays which use 32P-labelled probes for the routine detection of infectious virus particles both in blood and in other biological fluids.

Original languageEnglish
Pages (from-to)339-342
Number of pages4
JournalJournal of Virological Methods
Volume16
Issue number4
DOIs
Publication statusPublished - 1987

Keywords

  • Biotin
  • DNA
  • HBV
  • Hybridisation
  • Serum

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