TY - JOUR
T1 - Vernonia kotschyana roots
T2 - Therapeutic potential via antioxidant activity
AU - Vasincu, Alexandru
AU - Paulsen, Berit S.
AU - Diallo, Drissa
AU - Vasincu, Ioana
AU - Aprotosoaie, Ana C.
AU - Bild, Veronica
AU - Charalambous, Christiana
AU - Constantinou, Andreas I.
AU - Miron, Anca
AU - Gavrilescu, Cristina M.
PY - 2014/11/1
Y1 - 2014/11/1
N2 - The roots of Vernonia kotschyana Sch. Bip. ex Walp. (Asteraceae) are used in Malian traditional medicine in the treatment of gastroduodenal ulcers and gastritis. Since oxidative stress is involved in gastric ulceration, the aim of this study was to screen the root extracts for their in vitro antioxidant activity and phenolic content. The roots were extracted successively with chloroform, ethyl acetate, ethanol and water. The antioxidant activity of root extracts was evaluated in both cell-free and cell-based assays. Their chemical characterization was performed by Fourier transform infrared spectroscopy (FT-IR) whereas the total phenolic content was determined by the Folin-Ciocalteu method. The ethyl acetate extract displayed the highest phenolic content and was found to be the most active in the free radical scavenging and lipid peroxidation inhibition assays; it also showed a high antioxidant activity in MCF-12F cells. This study suggests a potential use of the ethyl acetate extract of Vernonia kotschyana not only as an antioxidant agent in gastroduodenal ulcers and gastritis, but also in other disorders characterized by high levels of oxidative stress.
AB - The roots of Vernonia kotschyana Sch. Bip. ex Walp. (Asteraceae) are used in Malian traditional medicine in the treatment of gastroduodenal ulcers and gastritis. Since oxidative stress is involved in gastric ulceration, the aim of this study was to screen the root extracts for their in vitro antioxidant activity and phenolic content. The roots were extracted successively with chloroform, ethyl acetate, ethanol and water. The antioxidant activity of root extracts was evaluated in both cell-free and cell-based assays. Their chemical characterization was performed by Fourier transform infrared spectroscopy (FT-IR) whereas the total phenolic content was determined by the Folin-Ciocalteu method. The ethyl acetate extract displayed the highest phenolic content and was found to be the most active in the free radical scavenging and lipid peroxidation inhibition assays; it also showed a high antioxidant activity in MCF-12F cells. This study suggests a potential use of the ethyl acetate extract of Vernonia kotschyana not only as an antioxidant agent in gastroduodenal ulcers and gastritis, but also in other disorders characterized by high levels of oxidative stress.
KW - Ethyl acetate extract
KW - Free radical scavenging activity
KW - FT-IR spectroscopy
KW - Lipid peroxidation inhibition
KW - Phenolic content
KW - Vernonia kotschyana
UR - http://www.scopus.com/inward/record.url?scp=84911999093&partnerID=8YFLogxK
U2 - 10.3390/molecules191119114
DO - 10.3390/molecules191119114
M3 - Article
C2 - 25415475
AN - SCOPUS:84911999093
SN - 1420-3049
VL - 19
SP - 19114
EP - 19136
JO - Molecules
JF - Molecules
IS - 11
ER -